RGD Reference Report - Distribution of organic anion-transporting polypeptide 2 (oatp2) and oatp3 in the rat retina. - Rat Genome Database

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Distribution of organic anion-transporting polypeptide 2 (oatp2) and oatp3 in the rat retina.

Authors: Ito, A  Yamaguchi, K  Onogawa, T  Unno, M  Suzuki, T  Nishio, T  Suzuki, Takashi  Sasano, Hironobu  Abe, Takaaki  Tamai, Makoto 
Citation: Ito A, etal., Invest Ophthalmol Vis Sci 2002 Mar;43(3):858-63.
RGD ID: 70527
Pubmed: PMID:11867608   (View Abstract at PubMed)

PURPOSE: To examine the expression of multifunctional Na(+)-independent organic anion-transporting polypeptides, termed oatp1, oatp2, and oatp3, involving the transport of thyroid hormone in the rat retina at the protein and mRNA levels. METHODS: Northern blot analysis was performed using oatp1, -2, and -3 cDNAs. Reverse transcription-polymerase chain reaction (RT-PCR) was also performed using gene-specific primers for oatp1, -2, and -3. mRNA distribution of these oatps in the rat retina was examined by in situ hybridization. Western blot analysis and immunohistochemistry were also performed by raising specific antibodies against oatp2 and -3. RESULTS: Northern blot analysis showed that the mRNAs for oatp2 and -3 were expressed in the rat retina and retinal pigment epithelium (RPE). Amplified cDNA products by RT-PCR for oatp2 and -3 were also detected in the rat retina-RPE. In contrast, no specific band for oatp1 was detected by Northern blot analysis or RT-PCR. By in situ hybridization, oatp2-specific mRNA signals were seen in the RPE and inner nuclear layer, whereas the oatp3 mRNA signal was localized to the ganglion cell. At the protein level, a single band for oatp2 and -3 proteins was detected in the rat retina-RPE by Western blot analysis. Immunohistochemistry revealed that oatp2 immunostaining was predominantly expressed at the apical surface of the RPE. Weak immunostaining for oatp2 was also seen in the inner nuclear layer and the ganglion cell layer. In contrast, apparent immunostaining for oatp3 was seen in the nerve fiber layer, ganglion cell layer, inner plexiform layer, and outer aspect of the inner nuclear layer. In addition, oatp3 immunostaining was detected predominantly in the optic nerve fiber. CONCLUSIONS: These results reveal that oatp2 is localized mainly in the RPE, suggesting a role for organic anion transport in this specialized ocular tissue. In contrast, oatp3 is localized mainly in optic nerve fibers, suggesting that oatp3 is a specific transporter in the visual nervous system. In conclusion, these data suggest that oatp2 and -3 may be involved in the transport of thyroid hormone in the rat retina.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Slco1a1Ratorganic anion transport  TAS  RGD 
Slco1a5Ratorganic anion transport  TAS  RGD 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Slco1a1Ratorganic anion transmembrane transporter activity  TAS  RGD 
Slco1a5Ratorganic anion transmembrane transporter activity  TAS  RGD 

Molecular Pathway Annotations    Click to see Annotation Detail View

RGD Manual Annotations


  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Slco1a5Ratmultispecific organic anion transporter mediated transport pathway  TAS  RGD 
Objects Annotated

Genes (Rattus norvegicus)
Slco1a1  (solute carrier organic anion transporter family, member 1a1)
Slco1a5  (solute carrier organic anion transporter family, member 1A5)

Objects referenced in this article
Gene Slco1c1 solute carrier organic anion transporter family, member 1c1 Rattus norvegicus

Additional Information