RGD Reference Report - Expression analysis of Notch-related molecules in peripheral blood T helper cells of patients with rheumatoid arthritis. - Rat Genome Database

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Expression analysis of Notch-related molecules in peripheral blood T helper cells of patients with rheumatoid arthritis.

Authors: Jiao, Z  Wang, W  Guo, M  Zhang, T  Chen, L  Wang, Y  You, H  Li, J 
Citation: Jiao Z, etal., Scand J Rheumatol. 2010;39(1):26-32.
RGD ID: 6480790
Pubmed: PMID:20132067   (View Abstract at PubMed)
DOI: DOI:10.3109/03009740903124424   (Journal Full-text)

OBJECTIVE: Expression of Notch homologues in local tissue inflammation in rheumatoid arthritis (RA) and cultured synoviocytes has been reported, but the expression profile of Notch-related molecules in peripheral lymphocytes in RA remains unclear. In this study, we measured the expression of Notch receptors and downstream molecules in peripheral lymphocytes from RA patients. METHODS: Expression of Notch receptors in peripheral lymphocytes of RA patients was assessed by both flow cytometry and real-time polymerase chain reaction (PCR). Expression of the representative Notch target gene HES-1 and the regulatory gene NUMB in purified T helper cells from RA patients was determined by real-time PCR, and expression of Notch intracellular domain (ICD) was determined by immunoblot analysis. RESULTS: There was an increased expression of Notch 2, Notch 3, and Notch 4 in T helper cells from active RA patients, among which increased expression of Notch 3 was mainly by activated T cells. Notably, expression of Notch 3 in T cells decreased in inactive RA patients and the level was similar to that of healthy controls (HC). Notch receptors were rarely observed on B cells and no difference in expression was found between RA patients and HC. T helper cells from RA patients exhibited increased expression of the target gene HES-1 but decreased expression of the negative modulation gene NUMB of Notch signalling. There was also an increased nuclear translocation of Notch-ICD in T helper cells from active RA disease. CONCLUSION: The present study demonstrated that T helper cells from RA patients display a significantly altered expression profile of Notch receptors and enhanced activation of Notch signalling compared with HC.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
NOTCH4Humanrheumatoid arthritis  IEP mRNA : increased expression: : cd4+ cellsRGD 
Notch4Ratrheumatoid arthritis  ISONOTCH4 (Homo sapiens)mRNA : increased expression: : cd4+ cellsRGD 
Notch4Mouserheumatoid arthritis  ISONOTCH4 (Homo sapiens)mRNA : increased expression: : cd4+ cellsRGD 

Objects Annotated

Genes (Rattus norvegicus)
Notch4  (notch receptor 4)

Genes (Mus musculus)
Notch4  (notch 4)

Genes (Homo sapiens)
NOTCH4  (notch receptor 4)


Additional Information