RGD Reference Report - Expression and localization of a novel Rab small G protein (Rab38) in the rat lung. - Rat Genome Database

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Expression and localization of a novel Rab small G protein (Rab38) in the rat lung.

Authors: Osanai, K  Iguchi, M  Takahashi, K  Nambu, Y  Sakuma, T  Toga, H  Ohya, N  Shimizu, H  Fisher, JH  Voelker, DR 
Citation: Osanai K, etal., Am J Pathol 2001 May;158(5):1665-75.
RGD ID: 633808
Pubmed: PMID:11337364   (View Abstract at PubMed)
PMCID: PMC1891947   (View Article at PubMed Central)
DOI: DOI:10.1016/S0002-9440(10)64122-3   (Journal Full-text)

The Rab small G protein family participates in intracellular vesicle transport, including exocytosis and endocytosis. The cDNA encoding a novel Rab-related small G protein (Rab38) has been cloned from rat lung cDNA library and recorded in GenBank (accession no. M94043). However, the expression and localization of the protein in the lung remains primarily unknown. We produced polyhistidine-tagged recombinant Rab38 and a polyclonal antibody with a synthetic peptide. Immunohistochemistry demonstrated that the protein is specifically localized in alveolar type II cells and in bronchial epithelial cells. In situ hybridization using a digoxygenin-labeled RNA riboprobe clearly showed that the mRNA of the protein is localized in alveolar type II cells and bronchial epithelial cells, especially terminal airway epithelial cells. Western blot and reverse transcriptase-polymerase chain reaction showed distinct expression of the protein and mRNA in isolated alveolar type II cells, but not in alveolar macrophages. The native protein was predominantly hydrophobic and was enriched in a high-density vesicle fraction but was barely detectable in nuclear and lamellar body fractions in alveolar type II cells. Immunofluorescence cytochemistry performed on cultured alveolar type II cells showed that Rab38 distributed extensively in the cytoplasm with a distribution pattern similar to endoplasmic reticulum rather than other subcellular organelles. These results suggest that this novel rab small G protein (Rab38) mediates vesicular transport in terminal airway epithelium.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
vesicle-mediated transport  NAS 633808 RGD 

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
vesicle  IDA 633808 RGD 

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
GTP binding  TAS 633808 RGD 
GTPase activity  TAS 633808 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Rab38  (RAB38, member RAS oncogene family)


Additional Information