RGD Reference Report - Synergistic activation of the rat laminin gamma1 chain promoter by the gut-enriched Kruppel-like factor (GKLF/KLF4) and Sp1. - Rat Genome Database

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Synergistic activation of the rat laminin gamma1 chain promoter by the gut-enriched Kruppel-like factor (GKLF/KLF4) and Sp1.

Authors: Higaki, Y  Schullery, D  Kawata, Y  Shnyreva, M  Abrass, C  Bomsztyk, K 
Citation: Higaki Y, etal., Nucleic Acids Res 2002 Jun 1;30(11):2270-9.
RGD ID: 619591
Pubmed: PMID:12034813   (View Abstract at PubMed)
PMCID: PMC117209   (View Article at PubMed Central)

Laminin is a multifunctional heterotrimeric protein present in extracellular matrix where it regulates processes that compose tissue architecture including cell differentiation. Laminin gamma1 is the most widely expressed laminin chain and its absence causes early lethality in mouse embryos. Laminin gamma1 chain gene (LAMC1) promoter contains several GC/GT-rich motifs including the bcn-1 element. Using the bcn-1 element as a bait in the yeast one-hybrid screen, we cloned the gut-enriched Kruppel-like factor (GKLF or KLF4) from a rat mesangial cell library. We show that GKLF binds bcn-1, but this binding is not required for the GKLF-mediated activation of the LAMC1 promoter. The activity of GKLF is dependent on a synergism with another Kruppel-like factor, Sp1. The LAMC1 promoter appears to have multiple GKLF- and Sp1-responsive elements which may account for the synergistic activation. We provide evidence that the synergistic action of GKLF and Sp1 is dependent on the promoter context and the integrity of GKLF activation and DNA-binding domain. GKLF is thought to participate in the switch from cell proliferation to differentiation. Thus, the Sp1-GKLF synergistic activation of the LAMC1 promoter may be one of the avenues for expression of laminin gamma1 chain when laminin is needed to regulate cell differentiation.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Klf4Ratpositive regulation of transcription by RNA polymerase II  IDA  RGD 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Klf4RatDNA-binding transcription factor activity  IDA  RGD 
Klf4Ratdouble-stranded DNA binding  IDA  RGD 
Klf4Ratsequence-specific DNA binding  IDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Klf4  (KLF transcription factor 4)

Objects referenced in this article
Gene Lamc1 laminin subunit gamma 1 Rattus norvegicus
Gene Sp1 Sp1 transcription factor Rattus norvegicus

Additional Information