RGD Reference Report - Identification of HSP90 as a new GABARAPL1 (GEC1)-interacting protein. - Rat Genome Database

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Identification of HSP90 as a new GABARAPL1 (GEC1)-interacting protein.

Authors: Seguin-Py, S  Lucchi, G  Croizier, S  Chakrama, FZ  Despouy, G  Le Grand, JN  Ducoroy, P  Boireau, W  Boyer-Guittaut, M  Jouvenot, M  Fraichard, A  Delage-Mourroux, R 
Citation: Seguin-Py S, etal., Biochimie. 2011 Nov 20.
RGD ID: 5686376
Pubmed: PMID:22120110   (View Abstract at PubMed)
DOI: DOI:10.1016/j.biochi.2011.11.006   (Journal Full-text)

GABARAPL1 belongs to the small family of GABARAP proteins (including GABARAP, GABARAPL1 and GABARAPL2/GATE-16), one of the two subfamilies of the yeast Atg8 orthologue. GABARAPL1 is involved in the intracellular transport of receptors, via an interaction with tubulin and GABA(A) or kappa opioid receptors, and also participates in autophagy and cell proliferation. In the present study, we identify the HSP90 protein as a novel interaction partner for GABARAPL1 using GST pull-down, mass spectrometry and coimmunoprecipitation experiments. GABARAPL1 and HSP90 partially colocalize in MCF-7 breast cancer cells overexpressed Dsred-GABARAPL1 and in rat brain. Moreover, treatment of MCF-7 cells overexpressed FLAG-GABARAPL1-6HIS with the HSP90 inhibitor 17-AAG promotes the GABARAPL1 degradation, a process that is blocked by proteasome inhibitors such as MG132, bortezomib and lactacystin. Accordingly, we demonstrate that HSP90 interacts and protects GABARAPL1 from its degradation by the proteasome.




Molecular Function

  
Object Symbol
Species
Term
Qualifier
Evidence
With
Notes
Source
Original Reference(s)
Gabarapl1Ratprotein binding  IPIHsp90ab1 (Rattus norvegicus) RGD 
Hsp90ab1Ratprotein binding  IPIGabarapl1 (Rattus norvegicus) RGD 


Genes (Rattus norvegicus)
Gabarapl1  (GABA type A receptor associated protein like 1) Hsp90ab1  (heat shock protein 90 alpha family class B member 1)