RGD Reference Report - MicroRNA Expression in Induced Sputum of Smokers and Patients with Chronic Obstructive Pulmonary Disease. - Rat Genome Database

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MicroRNA Expression in Induced Sputum of Smokers and Patients with Chronic Obstructive Pulmonary Disease.

Authors: Pottelberge, GR  Mestdagh, P  Bracke, KR  Thas, O  Durme, YM  Joos, GF  Vandesompele, J  Brusselle, GG 
Citation: Pottelberge GR, etal., Am J Respir Crit Care Med. 2011 Apr 1;183(7):898-906. Epub 2010 Oct 29.
RGD ID: 5131154
Pubmed: PMID:21037022   (View Abstract at PubMed)
DOI: DOI:10.1164/rccm.201002-0304OC   (Journal Full-text)

Rationale: Chronic obstructive pulmonary disease (COPD) is characterized by progressive inflammation in the airways and lungs combined with disturbed homeostatic functions of pulmonary cells. MicroRNAs (miRNAs) have the ability to regulate these processes by interfering with gene transcription and translation. Objectives: We aimed to identify miRNA expression in induced sputum and examined whether the expression of miRNAs differed between patients with COPD and subjects without airflow limitation. Methods: Expression of 627 miRNAs was evaluated in induced sputum supernatant of 32 subjects by stem-loop reverse transcription-quantitative polymerase chain reaction. Differentially expressed miRNAs were validated in an independent replication cohort of 41 subjects. Enrichment of miRNA target genes was identified by in silico analysis. Protein expression of target genes was determined by ELISA. Measurements and Main Results: Thirty-four miRNAs were differentially expressed between never-smokers and current smokers without airflow limitation in the screening cohort. Eight miRNAs were expressed at a significantly lower level in current-smoking patients with COPD compared with never-smokers without airflow limitation. Reduced expression of let-7c and miR-125b in patients with COPD compared with healthy subjects was confirmed in the validation cohort. Target genes of let-7c were significantly enriched in the sputum of patients with severe COPD. The concentration of tumor necrosis factor receptor type II (TNFR-II, implicated in COPD pathogenesis and a predicted target gene of let-7c) was inversely correlated with the sputum levels of let-7c . Conclusions: let-7c is significantly reduced in the sputum of currently smoking patients with COPD and is associated with increased expression of TNFR-II.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
TNFRSF1BHumanchronic obstructive pulmonary disease severityIEP protein:increased expression:sputum (human)RGD 
Tnfrsf1bRatchronic obstructive pulmonary disease severityISOTNFRSF1B (Homo sapiens)protein:increased expression:sputum (human)RGD 
Tnfrsf1bMousechronic obstructive pulmonary disease severityISOTNFRSF1B (Homo sapiens)protein:increased expression:sputum (human)RGD 

Objects Annotated

Genes (Rattus norvegicus)
Tnfrsf1b  (TNF receptor superfamily member 1B)

Genes (Mus musculus)
Tnfrsf1b  (tumor necrosis factor receptor superfamily, member 1b)

Genes (Homo sapiens)
TNFRSF1B  (TNF receptor superfamily member 1B)


Additional Information