RGD Reference Report - Rat C-reactive protein activates the autologous complement system. - Rat Genome Database

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Rat C-reactive protein activates the autologous complement system.

Authors: Diaz Padilla, N  Bleeker, WK  Lubbers, Y  Rigter, GM  Van Mierlo, GJ  Daha, MR  Hack, CE 
Citation: Diaz Padilla N, etal., Immunology. 2003 Aug;109(4):564-71.
RGD ID: 5129545
Pubmed: PMID:12871224   (View Abstract at PubMed)
PMCID: PMC1783000   (View Article at PubMed Central)

Activation of complement is a biological function of human C-reactive protein (hCRP), whereas rat CRP (rCRP) has been claimed to be unable to activate complement. As important biological functions of proteins are probably conserved among species, we re-evaluated, using various ligands, the capability of rCRP to activate complement. The activation of complement by hCRP and rCRP was investigated in solid- and fluid-phase systems. In the solid-phase system, purified CRP was fixed to enzyme-linked immunosorbent assay (ELISA) plates and incubated with human or rat recalcified plasma. Dose-dependent binding of human and rat C3 and C4 was observed to human and rat CRP, respectively. In the fluid-phase system, recalcified rat plasma, which contains about 500 mg/l of CRP, or human plasma supplemented with hCRP, were incubated with lyso-phosphatidylcholine. A dose-dependent activation of complement was observed upon incubation with this ligand, as reflected by the generation of activated C4 as well as of CRP-complement complexes. This activation was, in both cases, inhibited by preincubation of plasma with p-aminophosphorylcholine, a specific inhibitor of the interaction of CRP with its ligands, or by chelation of calcium ions. We conclude that rat CRP, similarly to human CRP, can activate autologous complement. These results support the notion that opsonization of ligands with complement is an important biological function of CRP.



Gene Ontology Annotations    Click to see Annotation Detail View

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
C3Ratprotein binding  IPICrp (Rattus norvegicus) RGD 
CrpRatprotein binding  IPIC3 (Rattus norvegicus) RGD 

Objects Annotated

Genes (Rattus norvegicus)
C3  (complement C3)
Crp  (C-reactive protein)


Additional Information