RGD Reference Report - Noradrenergic innervation of hypophysiotropic thyrotropin-releasing hormone-synthesizing neurons in rats. - Rat Genome Database

Send us a Message
Submit Data |  Help |  Video Tutorials |  News |  Publications |  Download |  REST API |  Citing RGD |  Contact   
Search RGD Grant Resources Citing RGD About Us Contact Us
Genes Variants Community Projects QTLs Strains Markers Genome Information Ontologies Cell Lines References Download Submit Data
OntoMate (Literature Search) JBrowse (Genome Browser) Synteny Browser (VCMap) Variant Visualizer Multi-Ontology Enrichment (MOET) Gene-Ortholog Location Finder (GOLF) InterViewer (Protein-Protein Interactions) PhenoMiner (Quatitative Phenotypes) Gene Annotator OLGA (Gene List Generator) AllianceMine GViewer (Genome Viewer)
Aging & Age-Related Disease Cancer & Neoplastic Disease Cardiovascular Disease Coronavirus Disease Developmental Disease Diabetes Hematologic Disease Immune & Inflammatory Disease Infectious Disease Liver Disease Neurological Disease Obesity & Metabolic Syndrome Renal Disease Respiratory Disease Sensory Organ Disease
Find Models Genetic Models Autism Models Rat PhenoMiner (Quantitative Phenotypes) Chinchilla PhenoMiner Expected Ranges (Quantitative Phenotype) PhenoMiner Term Comparison Hybrid Rat Diversity Panel Phenotypes Phenotypes in Other Animal Models Animal Husbandry Strain Medical Records Phylogenetics Strain Availability Calendar Rats 101 Submissions Photo Archive
Pathways
Rat Community Forum Directory of Rat Laboratories Video Tutorials News RGD Publications RGD Presentations Archive Nomenclature Guidelines Resource Links Laboratory Resources Employment Resources
Advanced Search (OLGA)

Noradrenergic innervation of hypophysiotropic thyrotropin-releasing hormone-synthesizing neurons in rats.

Authors: Fuzesi, T  Wittmann, G  Lechan, RM  Liposits, Z  Fekete, C 
Citation: Fuzesi T, etal., Brain Res. 2009 Oct 19;1294:38-44. Epub 2009 Aug 6.
RGD ID: 5129482
Pubmed: PMID:19651110   (View Abstract at PubMed)
PMCID: PMC2764741   (View Article at PubMed Central)
DOI: DOI:10.1016/j.brainres.2009.07.094   (Journal Full-text)

Hypophysiotropic thyrotropin-releasing hormone (TRH)-synthesizing neurons, the central regulators of the hypothalamic-pituitary-thyroid axis, are located in the paraventricular nucleus of the hypothalamus (PVN). These neurons are well-known to be stimulated by cold exposure through activation of ascending brainstem pathways, and are heavily innervated by catecholaminergic axons that contain dopamine-beta-hydroxylase (DBH) and phenylethanolamine-N-methyltransferase (PNMT), enzymes that generate noradrenaline and adrenaline, respectively. However, whether noradrenergic cell groups that lack PNMT contribute to the innervation of TRH neurons is not known. Therefore, triple-labeling immunofluorescence was performed using antibodies against DBH, PNMT and proTRH to determine the relative involvement of adrenaline-synthesizing and noradrenergic neurons in the innervation of TRH neurons in the PVN of rats. Using confocal microscopy, the number of PNMT/DBH (adrenaline-synthesizing) and single-labeled DBH (noradrenergic) boutons juxtaposed to proTRH neurons was quantified. Both noradrenergic and PNMT-containing varicosities were observed in close apposition to virtually all proTRH neurons. An average of 11.8+/-0.6 PNMT-containing and 7.4+/-1.0 noradrenergic boutons was present on the surface of proTRH cell bodies and proximal dendrites. Of all catecholaminergic axon-varicosities juxtaposed to proTRH neurons, 63.5+/-1.2% contained PNMT while the remaining 36.5+/-1.2% were immunopositive for DBH only. We conclude that both adrenaline-synthesizing and noradrenergic axons innervate hypophysiotropic TRH neurons, although there is a predominance of adrenaline-synthesizing fibers. Since adrenaline-synthesizing and noradrenergic cell groups of the brainstem may respond differently to various physiological stimuli, we hypothesize that the two cell groups are likely to mediate the effects of distinct stimuli toward the hypophysiotropic TRH neurons.



Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
PnmtRataxon  IDA  RGD 
DbhRatterminal bouton  IDA  RGD 
PnmtRatterminal bouton  IDA  RGD 
DbhRatvaricosity  IDA  RGD 
PnmtRatvaricosity  IDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Dbh  (dopamine beta-hydroxylase)
Pnmt  (phenylethanolamine-N-methyltransferase)


Additional Information