RGD Reference Report - Over expression of proteins that alter the intracellular signaling pathways in the cytoplasm of the liver cells forming Mallory-Denk bodies. - Rat Genome Database

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Over expression of proteins that alter the intracellular signaling pathways in the cytoplasm of the liver cells forming Mallory-Denk bodies.

Authors: Afifiyan, N  Tillman, B  French, B A  Masouminia, M  Samadzadeh, S  French, S W 
Citation: Afifiyan N, etal., Exp Mol Pathol. 2017 Feb;102(1):106-114. doi: 10.1016/j.yexmp.2017.01.011. Epub 2017 Jan 13.
RGD ID: 407987426
Pubmed: PMID:28089901   (View Abstract at PubMed)
PMCID: PMC5295648   (View Article at PubMed Central)
DOI: DOI:10.1016/j.yexmp.2017.01.011   (Journal Full-text)

In this study, liver biopsy sections fixed in formalin and embedded in paraffin (FFPE) from patients with alcoholic hepatitis (AH) were used. The results showed that the expression of the SYK protein was up regulated by RNA-seq and real time PCR analyses in the alcoholic hepatitis patients compared to controls. The results were supported by using the IHC fluorescent antibody staining intensity morphometric quantitation. Morphometric quantification of fluorescent intensity measurement showed a two fold increase in SYK protein in the cytoplasm of the cells forming MDBs compared to surrounding normal hepatocytes. The expression of AKT1 was also analyzed. AKT1 is a serine/threonine-specific protein kinase that plays a key role in multiple cellular processes such as glucose metabolism, apoptosis, cell proliferation, transcription and cell migration. The AKT protein was also increased in hepatocyte balloon cells forming MDBs. This observation demonstrates the role of SYK and its subsequent effect on the internal signaling pathways such as PI3K/AKT as well as p70S6K, as a potential multifunctional target in protein quality control mechanisms of hepatocytes when ER stress is activated.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
AKT1Humanalcoholic hepatitis  IEP protein:increased expression:liverRGD 
Akt1Ratalcoholic hepatitis  ISOAKT1 (Homo sapiens)protein:increased expression:liverRGD 
Akt1Mousealcoholic hepatitis  ISOAKT1 (Homo sapiens)protein:increased expression:liverRGD 

Objects Annotated

Genes (Rattus norvegicus)
Akt1  (AKT serine/threonine kinase 1)

Genes (Mus musculus)
Akt1  (thymoma viral proto-oncogene 1)

Genes (Homo sapiens)
AKT1  (AKT serine/threonine kinase 1)


Additional Information