RGD Reference Report - Self administration of oxycodone alters synaptic plasticity gene expression in the hippocampus differentially in male adolescent and adult mice. - Rat Genome Database

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Self administration of oxycodone alters synaptic plasticity gene expression in the hippocampus differentially in male adolescent and adult mice.

Authors: Zhang, Y  Brownstein, A J  Buonora, M  Niikura, K  Ho, A  Correa da Rosa, J  Kreek, M J  Ott, J 
Citation: Zhang Y, etal., Neuroscience. 2015 Jan 29;285:34-46. doi: 10.1016/j.neuroscience.2014.11.013. Epub 2014 Nov 14.
RGD ID: 407445976
Pubmed: PMID:25446355   (View Abstract at PubMed)
DOI: DOI:10.1016/j.neuroscience.2014.11.013   (Journal Full-text)

Abuse and addiction to prescription opioids such as oxycodone (a short-acting Mu opioid receptor (MOP-r) agonist) in adolescence is a pressing public health issue. We have previously shown differences in oxycodone self-administration behaviors between adolescent and adult C57BL/6J mice and expression of striatal neurotransmitter receptor genes, in areas involved in reward. In this study, we aimed to determine whether oxycodone self-administration differentially affects genes regulating synaptic plasticity in the hippocampus of adolescent compared to adult mice, since the hippocampus may be involved in learning aspects associated with chronic drug self administration. Hippocampus was isolated for mRNA analysis from mice that had self administered oxycodone (0.25 mg/kg/infusion) 2h/day for 14 consecutive days or from yoked saline controls. Gene expression was analyzed with real-time polymerase chain reaction (PCR) using a commercially available "synaptic plasticity" PCR array containing 84 genes. We found that adolescent and adult control mice significantly differed in the expression of several genes in the absence of oxycodone exposure, including those coding for mitogen-activated protein kinase, calcium/calmodulin-dependent protein kinase II gamma subunit, glutamate receptor, ionotropic AMPA2 and metabotropic 5. Chronic oxycodone self administration increased proviral integration site 1 (Pim1) and thymoma viral proto-oncogene 1 mRNA levels compared to controls in both age groups. Both Pim1 and cadherin 2 mRNAs showed a significant combined effect of Drug Condition and Age × Drug Condition. Furthermore, the mRNA levels of both cadherin 2 and cAMP response element modulators showed an experiment-wise significant difference between oxycodone and saline control in adult but not in adolescent mice. Overall, this study demonstrates for the first time that chronic oxycodone self-administration differentially alters synaptic plasticity gene expression in the hippocampus of adolescent and adult mice.



Gene-Chemical Interaction Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
AKT1Humanoxycodone increases expression ISOAkt1 (Mus musculus)Oxycodone increases expression of Akt1 mRNA in the hippocampusRGD 
Akt1Ratoxycodone increases expression ISOAkt1 (Mus musculus)Oxycodone increases expression of Akt1 mRNA in the hippocampusRGD 
Akt1Mouseoxycodone increases expression EXP Oxycodone increases expression of Akt1 mRNA in the hippocampusRGD 

Objects Annotated

Genes (Rattus norvegicus)
Akt1  (AKT serine/threonine kinase 1)

Genes (Mus musculus)
Akt1  (thymoma viral proto-oncogene 1)

Genes (Homo sapiens)
AKT1  (AKT serine/threonine kinase 1)


Additional Information