RGD Reference Report - Modulation of potassium channel gating by coexpression of Kv2.1 with regulatory Kv5.1 or Kv6.1 alpha-subunits. - Rat Genome Database

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Modulation of potassium channel gating by coexpression of Kv2.1 with regulatory Kv5.1 or Kv6.1 alpha-subunits.

Authors: Kramer, J W  Post, M A  Brown, A M  Kirsch, G E 
Citation: Kramer JW, etal., Am J Physiol. 1998 Jun;274(6):C1501-10. doi: 10.1152/ajpcell.1998.274.6.C1501.
RGD ID: 405866205
Pubmed: PMID:9696692   (View Abstract at PubMed)
DOI: DOI:10.1152/ajpcell.1998.274.6.C1501   (Journal Full-text)

We have determined the effects of coexpression of Kv2.1 with electrically silent Kv5.1 or Kv6.1 alpha-subunits in Xenopus oocytes on channel gating. Kv2.1/5.1 selectively accelerated the rate ofinactivation at intermediate potentials (-30 to 0 mV), without affecting the rate at strong depolarization (0 to +40 mV), and markedly accelerated the rate of cumulative inactivation evoked by high-frequency trains of short pulses. Kv5.1 coexpression alsoslowed deactivation of Kv2.1. In contrast, Kv6.1 was much less effective in speeding inactivation at intermediate potentials, had a slowing effect on inactivation at strong depolarizations, and had no effect on cumulative inactivation. Kv6.1, however, had profound effects on activation, including a negative shift of the steady-state activation curve and marked slowing of deactivation tail currents. Support for the notion that the Kv5.1's effects stem from coassembly of alpha-subunits into heteromeric channels was obtained from biochemical evidence of protein-protein interaction and single-channel measurements that showed heterogeneity in unitary conductance. Our results show that Kv5.1 and Kv6.1 function as regulatory alpha-subunits that when coassembled with Kv2.1 can modulate gating in a physiologically relevant manner.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Kcnf1Ratregulation of potassium ion transport involved_inIDA PMID:9696692UniProt 
Kcng1Ratregulation of potassium ion transport involved_inIDA PMID:9696692UniProt 

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Kcnb1Ratvoltage-gated potassium channel complex part_ofIDA PMID:9696692UniProt 
Kcnf1Ratvoltage-gated potassium channel complex part_ofIDA PMID:9696692UniProt 
Kcng1Ratvoltage-gated potassium channel complex part_ofIDA PMID:9696692UniProt 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Kcnf1Ratpotassium channel regulator activity enablesIDA PMID:9696692UniProt 
Kcng1Ratpotassium channel regulator activity enablesIDA PMID:9696692UniProt 

Objects Annotated

Genes (Rattus norvegicus)
Kcnb1  (potassium voltage-gated channel subfamily B member 1)
Kcnf1  (potassium voltage-gated channel modifier subfamily F member 1)
Kcng1  (potassium voltage-gated channel modifier subfamily G member 1)


Additional Information