RGD Reference Report - Synaptic activity and strength are reflected by changes in the post-synaptic secretory pathway. - Rat Genome Database

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Synaptic activity and strength are reflected by changes in the post-synaptic secretory pathway.

Authors: Gürth, Clara-Marie  Dankovich, Tal M  Rizzoli, Silvio O  D'Este, Elisa 
Citation: Gürth CM, etal., Sci Rep. 2020 Nov 25;10(1):20576. doi: 10.1038/s41598-020-77260-2.
RGD ID: 405650380
Pubmed: PMID:33239744   (View Abstract at PubMed)
PMCID: PMC7688657   (View Article at PubMed Central)
DOI: DOI:10.1038/s41598-020-77260-2   (Journal Full-text)

Neurons are highly asymmetric cells that span long distances and need to react promptly to local demands. Consequently, neuronal secretory pathway elements are distributed throughout neurites, specifically in post-synaptic compartments, to enable local protein synthesis and delivery. Whether and how changes in local synaptic activity correlate to post-synaptic secretory elements is still unclear. To assess this, we used STED nanoscopy and automated quantitative image analysis of post-synaptic markers of the endoplasmic reticulum, ER-Golgi intermediate compartment, trans-Golgi network, and spine apparatus. We found that the distribution of these proteins was dependent on pre-synaptic activity, measured as the amount of recycling vesicles. Moreover, their abundance correlated to both pre- and post-synaptic markers of synaptic strength. Overall, the results suggest that in small, low-activity synapses the secretory pathway components are tightly clustered in the synaptic area, presumably to enable rapid local responses, while bigger synapses utilise secretory machinery components from larger, more diffuse areas.



Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
CalrRatendoplasmic reticulum is_active_inIDA PMID:33239744SynGO 
CalrRatglutamatergic synapse is_active_inIDA PMID:33239744SynGO 
Lman1Ratglutamatergic synapse is_active_inIDA PMID:33239744SynGO 
Tgoln2Ratglutamatergic synapse is_active_inIDA PMID:33239744SynGO 
CalrRatpostsynapse is_active_inIDA PMID:33239744SynGO 
Lman1Ratpostsynaptic Golgi apparatus is_active_inIDA PMID:33239744SynGO 
Tgoln2Ratpostsynaptic Golgi apparatus is_active_inIDA PMID:33239744SynGO 

Objects Annotated

Genes (Rattus norvegicus)
Calr  (calreticulin)
Lman1  (lectin, mannose-binding, 1)
Tgoln2  (trans-golgi network protein 2)


Additional Information