RGD Reference Report - The effects of morphine treatment on the NCAM and its signaling in the MLDS of rats. - Rat Genome Database

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The effects of morphine treatment on the NCAM and its signaling in the MLDS of rats.

Authors: Cao, Jun Ping  Wang, Hong Jun  Li, Li  Zhang, Su Ming 
Citation: Cao JP, etal., Drug Chem Toxicol. 2016 Oct;39(4):418-23. doi: 10.3109/01480545.2015.1137302. Epub 2016 Jan 28.
RGD ID: 401959306
Pubmed: PMID:26821693   (View Abstract at PubMed)
DOI: DOI:10.3109/01480545.2015.1137302   (Journal Full-text)

Prolonged exposure to opiates induces a constellation of neuroadaptations, especially in the mesolimbic dopamine system (MLDS), which leads to alteration in the function of motivational circuitry. The neural cell adhesion molecule (NCAM) mediates cell-cell interactions and plays an important role in processes associated with neural plasticity. Moreover, it has been shown that NCAM were related to risk of alcoholism in human populations. Here, coimmunoprecipitation and western blotting were used to investigate whether morphine treatment induced alteration of the expression of NCAM or its signaling level in MLDS. The rats receiving escalating dose of morphine treatment were divided into three groups: morphine 1d, 3d and 5d group, which were injected subcutaneously with morphine hydrochloride for 1 day, 3 days and 5 days, respectively. Twelve hours after the last injection, animals were sacrificed and the tissues of ventral tegmental area (VTA), prefrontal cortex (PFC) and nucleus accumbens (NAc) were punched out to examine the expression of NCAM or its signaling level. The results showed that morphine treatment had no significant effect on the expression of NCAM, but downregulated the phosphorylation of NCAM-associated focal adhesion kinase (FAK) in the VTA and PFC of rats. In the NAc of rats, however, the expression of NCAM and its signaling were not altered significantly by morphine treatment. These results indicated that the downregulation of NCAM signaling in the VTA and PFC might be involved in the formation of morphine addiction.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Ptk2Ratcellular response to morphine  IEP  RGD 
Ncam1Ratregulation of protein phosphorylation  IDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Ncam1  (neural cell adhesion molecule 1)
Ptk2  (protein tyrosine kinase 2)


Additional Information