RGD Reference Report - Activation of NADPH oxidase and extracellular superoxide production in seizure-induced hippocampal damage. - Rat Genome Database

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Activation of NADPH oxidase and extracellular superoxide production in seizure-induced hippocampal damage.

Authors: Patel, M  Li, QY  Chang, LY  Crapo, J  Liang, LP 
Citation: Patel M, etal., J Neurochem. 2005 Jan;92(1):123-31.
RGD ID: 2314448
Pubmed: PMID:15606902   (View Abstract at PubMed)
DOI: DOI:10.1111/j.1471-4159.2004.02838.x   (Journal Full-text)

We sought to determine whether the extracellular compartment contributed to seizure-induced superoxide (O2*-) production and to determine the role of the NADPH oxidase complex as a source of this O2*- production. The translocation of NADPH oxidase subunits (p47phox, p67phox and rac1) was assessed by immunoblot analysis and NADPH-driven O2*- production was measured using 2-(4-hydroxybenzyl)-6-(4-hydroxyphenyl)-8-benzyl-3,7-dihydroimidazo [1,2-alpha] pyrazin-3-one-enhanced chemiluminescence. Kainate-induced status epilepticus resulted in a time-dependent translocation of NADPH oxidase subunits (p47phox, p67phox and rac-1) from hippocampal cytosol to membrane fractions. Hippocampal membrane fractions from kainate-injected rats showed increased NADPH-driven and diphenylene iodonium-sensitive O2*- production in comparison to vehicle-treated rats. The time-course of kainate-induced NADPH oxidase activation coincided with microglial activation in the rat hippocampus. Finally, kainate-induced neuronal damage and membrane oxygen consumption were inhibited in mice overexpressing extracellular superoxide dismutase. These results suggest that seizure activity activates the membrane NADPH oxidase complex resulting in increased formation of O2*-.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
NCF2Humanstatus epilepticus  ISONcf2 (Rattus norvegicus)associated with Experimental Seizures and protein:altered localizationRGD 
Ncf2Ratstatus epilepticus  IDA associated with Experimental Seizures and protein:altered localizationRGD 
Ncf2Mousestatus epilepticus  ISONcf2 (Rattus norvegicus)associated with Experimental Seizures and protein:altered localizationRGD 

Objects Annotated

Genes (Rattus norvegicus)
Ncf2  (neutrophil cytosolic factor 2)

Genes (Mus musculus)
Ncf2  (neutrophil cytosolic factor 2)

Genes (Homo sapiens)
NCF2  (neutrophil cytosolic factor 2)


Additional Information