RGD Reference Report - Changes in brain gene expression after long-term sleep deprivation. - Rat Genome Database

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Changes in brain gene expression after long-term sleep deprivation.

Authors: Cirelli, C  Faraguna, U  Tononi, G 
Citation: Cirelli C, etal., J Neurochem. 2006 Sep;98(5):1632-45.
RGD ID: 2313667
Pubmed: PMID:16923172   (View Abstract at PubMed)
DOI: DOI:10.1111/j.1471-4159.2006.04058.x   (Journal Full-text)

Long-term sleep deprivation in rats produces dramatic physiological changes including increase in energy expenditure, decrease in body weight, and death after 2-3 weeks. Despite several studies, the sleep deprivation syndrome remains largely unexplained. Here, to elucidate how prolonged sleep loss affects brain cells we used microarrays and screened the expression of > 26 000 transcripts in the cerebral cortex. Rats were sleep deprived using the disk-over-water method for 1 week. Seventy-five transcripts showed increased expression in these animals relative to controls that had been spontaneously awake or sleep deprived for a few hours. Most of them were induced as a result of chronic sleep loss and not non-specific effects of the disk stimulation. They include transcripts coding for several immunoglobulins, stress response proteins (macrophage inhibitor factor-related protein 14, heat-shock protein 27, alpha-B-crystallin), minoxidil sulfotransferase, globins and cortistatin. Twenty-eight transcripts decreased their expression in long-term sleep-deprived rats. Sixteen of them were specifically decreased as a result of chronic sleep loss, including those coding for type I procollagen and dihydrolipoamide acetyltransferase. We also compared sleeping rats to short-term and long-term sleep-deprived rats, and found that acute and chronic sleep loss led to some differences at the molecular level. Several plasticity-related genes were strongly induced after acute sleep deprivation only, and several glial genes were down-regulated in both sleep deprivation conditions, but to a different extent. These findings suggest that sustained sleep loss may trigger a generalized inflammatory and stress response in the brain.




  
Object Symbol
Species
Term
Qualifier
Evidence
With
Notes
Source
Original Reference(s)
DLATHumanSleep Deprivation  ISODlat (Rattus norvegicus)mRNA:decreased expression:cerebral cortexRGD 
DlatRatSleep Deprivation  IEP mRNA:decreased expression:cerebral cortexRGD 
DlatMouseSleep Deprivation  ISODlat (Rattus norvegicus)mRNA:decreased expression:cerebral cortexRGD 


Genes (Rattus norvegicus)
Dlat  (dihydrolipoamide S-acetyltransferase)

Genes (Mus musculus)
Dlat  (dihydrolipoamide S-acetyltransferase)

Genes (Homo sapiens)
DLAT  (dihydrolipoamide S-acetyltransferase)