RGD Reference Report - Physical and biochemical characterization of a purified arginyl-tRNA synthetase-lysyl-tRNA-synthetase complex from rat liver. - Rat Genome Database

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Physical and biochemical characterization of a purified arginyl-tRNA synthetase-lysyl-tRNA-synthetase complex from rat liver.

Authors: Van Dang, C  Glinski, RL  Gainey, PC  Hilderman, RH 
Citation: Van Dang C, etal., Biochemistry. 1982 Apr 13;21(8):1959-66.
RGD ID: 2303394
Pubmed: PMID:7082655   (View Abstract at PubMed)

Arginyl- and lysyl-tRNA synthetases copurify throughout a six-step chromatographic procedure resulting in a purification of 605- and 559-fold, respectively. The purified enzymes were estimated to be 98% pure with a stoichiometry of 1:1 from acrylamide gel electrophoresis under denaturing conditions. On the basis of a native molecular weight of 285000 calculated from s20,w, Rs, and V and subunit molecular weights of 73000 and 65000 obtained by sodium dodecyl sulfate gel electrophoresis, the synthetases appear to exist as a tetramer. The tetrameric structure was also supported by cross-linking studies. These results are consistent with an alpha 2 beta 2 structure, but an alpha beta structure has not been ruled out.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
arginyl-tRNA aminoacylation  IDA 2303394 RGD 
lysyl-tRNA aminoacylation  IDA 2303394 RGD 

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
aminoacyl-tRNA synthetase multienzyme complex  IDA 2303394; 2303394 RGD 

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
amino acid binding  IDA 2303394lysineRGD 
arginine binding  IDA 2303394 RGD 
arginine-tRNA ligase activity  IDA 2303394 RGD 
ATP binding  IDA 2303394; 2303394 RGD 
lysine-tRNA ligase activity  IDA 2303394 RGD 
tRNA binding  IDA 2303394; 2303394 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Kars1  (lysyl-tRNA synthetase 1)
Rars1  (arginyl-tRNA synthetase 1)


Additional Information