RGD Reference Report - Physical and biochemical characterization of a purified arginyl-tRNA synthetase-lysyl-tRNA-synthetase complex from rat liver. - Rat Genome Database

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Physical and biochemical characterization of a purified arginyl-tRNA synthetase-lysyl-tRNA-synthetase complex from rat liver.

Authors: Van Dang, C  Glinski, RL  Gainey, PC  Hilderman, RH 
Citation: Van Dang C, etal., Biochemistry. 1982 Apr 13;21(8):1959-66.
RGD ID: 2303394
Pubmed: PMID:7082655   (View Abstract at PubMed)

Arginyl- and lysyl-tRNA synthetases copurify throughout a six-step chromatographic procedure resulting in a purification of 605- and 559-fold, respectively. The purified enzymes were estimated to be 98% pure with a stoichiometry of 1:1 from acrylamide gel electrophoresis under denaturing conditions. On the basis of a native molecular weight of 285000 calculated from s20,w, Rs, and V and subunit molecular weights of 73000 and 65000 obtained by sodium dodecyl sulfate gel electrophoresis, the synthetases appear to exist as a tetramer. The tetrameric structure was also supported by cross-linking studies. These results are consistent with an alpha 2 beta 2 structure, but an alpha beta structure has not been ruled out.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Rars1Ratarginyl-tRNA aminoacylation  IDA  RGD 
Kars1Ratlysyl-tRNA aminoacylation  IDA  RGD 

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Kars1Rataminoacyl-tRNA synthetase multienzyme complex  IDA  RGD 
Rars1Rataminoacyl-tRNA synthetase multienzyme complex  IDA  RGD 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Kars1Ratamino acid binding  IDA lysineRGD 
Rars1Ratarginine binding  IDA  RGD 
Rars1Ratarginine-tRNA ligase activity  IDA  RGD 
Kars1RatATP binding  IDA  RGD 
Rars1RatATP binding  IDA  RGD 
Kars1Ratlysine-tRNA ligase activity  IDA  RGD 
Kars1RattRNA binding  IDA  RGD 
Rars1RattRNA binding  IDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Kars1  (lysyl-tRNA synthetase 1)
Rars1  (arginyl-tRNA synthetase 1)


Additional Information