RGD Reference Report - Quantitative promoter methylation analysis of multiple cancer-related genes in renal cell tumors. - Rat Genome Database

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Quantitative promoter methylation analysis of multiple cancer-related genes in renal cell tumors.

Authors: Costa, VL  Henrique, R  Ribeiro, FR  Pinto, M  Oliveira, J  Lobo, F  Teixeira, MR  Jeronimo, C 
Citation: Costa VL, etal., BMC Cancer. 2007 Jul 23;7:133.
RGD ID: 2299866
Pubmed: PMID:17645803   (View Abstract at PubMed)
PMCID: PMC1940017   (View Article at PubMed Central)
DOI: DOI:10.1186/1471-2407-7-133   (Journal Full-text)

BACKGROUND: Aberrant promoter hypermethylation of cancer-associated genes occurs frequently during carcinogenesis and may serve as a cancer biomarker. In this study we aimed at defining a quantitative gene promoter methylation panel that might identify the most prevalent types of renal cell tumors. METHODS: A panel of 18 gene promoters was assessed by quantitative methylation-specific PCR (QMSP) in 85 primarily resected renal tumors representing the four major histologic subtypes (52 clear cell (ccRCC), 13 papillary (pRCC), 10 chromophobe (chRCC), and 10 oncocytomas) and 62 paired normal tissue samples. After genomic DNA isolation and sodium bisulfite modification, methylation levels were determined and correlated with standard clinicopathological parameters. RESULTS: Significant differences in methylation levels among the four subtypes of renal tumors were found for CDH1 (p = 0.0007), PTGS2 (p = 0.002), and RASSF1A (p = 0.0001). CDH1 hypermethylation levels were significantly higher in ccRCC compared to chRCC and oncocytoma (p = 0.00016 and p = 0.0034, respectively), whereas PTGS2 methylation levels were significantly higher in ccRCC compared to pRCC (p = 0.004). RASSF1A methylation levels were significantly higher in pRCC than in normal tissue (p = 0.035). In pRCC, CDH1 and RASSF1A methylation levels were inversely correlated with tumor stage (p = 0.031) and nuclear grade (p = 0.022), respectively. CONCLUSION: The major subtypes of renal epithelial neoplasms display differential aberrant CDH1, PTGS2, and RASSF1A promoter methylation levels. This gene panel might contribute to a more accurate discrimination among common renal tumors, improving preoperative assessment and therapeutic decision-making in patients harboring suspicious renal masses.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
RASSF1Humanrenal cell carcinoma  IDA DNA:hypermethylation:promoterRGD 
Rassf1Ratrenal cell carcinoma  ISORASSF1 (Homo sapiens)DNA:hypermethylation:promoterRGD 
Rassf1Mouserenal cell carcinoma  ISORASSF1 (Homo sapiens)DNA:hypermethylation:promoterRGD 

Objects Annotated

Genes (Rattus norvegicus)
Rassf1  (Ras association domain family member 1)

Genes (Mus musculus)
Rassf1  (Ras association (RalGDS/AF-6) domain family member 1)

Genes (Homo sapiens)
RASSF1  (Ras association domain family member 1)


Additional Information