RGD Reference Report - Effects of hyperoxia on mitochondrial multienzyme complex III and V in premature newborn rat lung. - Rat Genome Database

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Effects of hyperoxia on mitochondrial multienzyme complex III and V in premature newborn rat lung.

Authors: Cai, C  Chang, L  Li, W  Liu, W 
Citation: Cai C, etal., J Huazhong Univ Sci Technolog Med Sci. 2008 Apr;28(2):207-10. Epub 2008 May 15.
RGD ID: 2298955
Pubmed: PMID:18481000   (View Abstract at PubMed)
DOI: DOI:10.1007/s11596-008-0224-4   (Journal Full-text)

To investigate the effects of hyperoxia on mitochondrial multienzyme complex III (cytochrome, Cytb) and V (ATPase6, 8) in premature newborn rat lung, the 1-day-old preterm SD rats were randomly assigned to hyperoxia group and air group. The rats in hyperoxia group were continuously exposed to 85% oxygen and those in air group to room air. After 1, 4, 7, 10, 14 day(s) of exposure, these rats were killed, total lung RNA was extracted and Cytb, ATPase6, 8 mRNA were detected by reverse transcription polymerase chain reaction (RT-PCR). Western blotting was used to detect the expression of Cytb protein in lung tissue. The results showed that compared with air group, Cytb mRNA expression was significantly increased (P>0.05) after 1, 4 day(s) of exposure. The general tendency decreased after 7 days, and its expression became weak but difference in mRNA expression between the two groups was not significant (P>0.05). ATPase6 mRNA expression was significantly increased 1 day after the exposure (P<0.05) and did not show any significant change 4, 7, 10 days after the exposure (P>0.05). At the 14th day, ATPase6 mRNA expression was significantly increased (P<0.05). ATPase8 mRNA expression did not show any significant change 1, 4, 10 day(s) after the exposure (P>0.05). At the 7th and 14th day, ATPase8 mRNA expression was significantly increased (P<0.05). Western blotting showed that Cytb protein expression was increased 1,4 day(s) after the exposure, but the difference between the two groups was not significant (P>0.05). The general tendency was decreased after 7 days, and its expression became weak but difference was not significant 7, 10 days after the exposure (P>0.05). At day 14 its expression became significantly weak (P<0.05). We are led to conclude that exposure to high concentrations of oxygen can significantly change the expression of Cytb and ATPase6, 8, which results in uncoupling of oxidative phosphorylation in mitochondrial respiration chain, and plays an important role in the mechanism of hyperoxia-induced lung injury.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
MT-CYBHumanHyperoxia  ISOMt-cyb (Rattus norvegicus)mRNA:increased expression:lungRGD 
Mt-cybRatHyperoxia  IEP mRNA:increased expression:lungRGD 
mt-CytbMouseHyperoxia  ISOMt-cyb (Rattus norvegicus)mRNA:increased expression:lungRGD 

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Mt-atp6Ratresponse to hyperoxia  IEP  RGD 
Mt-atp8Ratresponse to hyperoxia  IEP  RGD 
Mt-cybRatresponse to hyperoxia  IEP  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Mt-atp6  (mitochondrially encoded ATP synthase membrane subunit 6)
Mt-atp8  (mitochondrially encoded ATP synthase membrane subunit 8)
Mt-cyb  (mitochondrially encoded cytochrome b)

Genes (Mus musculus)
mt-Cytb  (cytochrome b, mitochondrial)

Genes (Homo sapiens)
MT-CYB  (mitochondrially encoded cytochrome b)


Additional Information