RGD Reference Report - Mitogen-activated protein kinase kinase 7 is activated during low potassium-induced apoptosis in rat cerebellar granule neurons. - Rat Genome Database

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Mitogen-activated protein kinase kinase 7 is activated during low potassium-induced apoptosis in rat cerebellar granule neurons.

Authors: Trotter, L  Panton, W  Hajimohamadreza, I  Petalidis, L  Ward, R  Fleming, Y  Armstrong, CG  Cohen, P  Karran, EH  Kinloch, RA 
Citation: Trotter L, etal., Neurosci Lett. 2002 Mar 1;320(1-2):29-32.
RGD ID: 2298565
Pubmed: PMID:11849756   (View Abstract at PubMed)

A stress-activated protein kinase pathway comprising mitogen-activated protein kinase kinases (MKKs), c-Jun N-terminal kinase (JNK) and the transcription factor c-Jun is implicated in neuronal apoptosis. Using an immune-complex kinase assay, we measured the activation of MKK4 and MKK7 in low potassium (LK)-induced apoptosis of rat cerebellar granule neurons (CGN). MKK7, but not MKK4, was activated within the first 4-6 h in four independent sets of 14-h CGN apoptosis time-courses. CEP-1347 (500 nM), an mixed-lineage kinase 3 inhibitor, prevented MKK7 activation and cell death following exposure of CGN cultures to LK-induced apoptosis. Western blot analysis revealed that levels of phosphorylated c-Jun were elevated between 30 min and 10 h of CGN apoptosis, temporally consistent with MKK7 activation. These data suggest that MKK7 is responsible for activating the JNK pathway during LK-induced CGN apoptosis.




Biological Process

  
Object Symbol
Species
Term
Qualifier
Evidence
With
Notes
Source
Original Reference(s)
Map2k7RatJNK cascade  IDA  RGD 
Map2k7Ratpositive regulation of JNK cascade  IDA  RGD 

Molecular Function

  
Object Symbol
Species
Term
Qualifier
Evidence
With
Notes
Source
Original Reference(s)
Mapk8RatJUN kinase activity  IDA  RGD 
Map2k7RatJUN kinase kinase activity  IDA  RGD 


Genes (Rattus norvegicus)
Map2k7  (mitogen activated protein kinase kinase 7) Mapk8  (mitogen-activated protein kinase 8)