RGD Reference Report - Altered PKC expression and phosphorylation in response to the nature, direction, and magnitude of mechanical stretch. - Rat Genome Database

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Altered PKC expression and phosphorylation in response to the nature, direction, and magnitude of mechanical stretch.

Authors: Bullard, TA  Hastings, JL  Davis, JM  Borg, TK  Price, RL 
Citation: Bullard TA, etal., Can J Physiol Pharmacol. 2007 Feb;85(2):243-50.
RGD ID: 1642539
Pubmed: PMID:17487266   (View Abstract at PubMed)
DOI: DOI:10.1139/y07-023   (Journal Full-text)

Protein kinase C (PKC) isozymes have been shown to play a role in mechanotransduction in a variety of cell types. We sought to identify the PKC isozymes involved in transducing mechanical (cyclic vs. static), direction and intensity of stretch by examining changes in protein expression and phosphorylation. We used a 3-dimensional culture system with aligned neonatal rat cardiac myocytes on silastic membranes. Myocytes were subjected to either cyclic stretch at 5 cycles/min or static stretch for a period of 24 h at intensities of 0%, 2.5%, 5%, or 10% of full membrane length. Stretch was applied in perpendicular or parallel directions to myocyte alignment. PKC delta was most sensitive to stretch applied perpendicular to myocyte alignment regardless of the nature of stretch, while phospho PKC delta T505 increased in response to static-perpendicular stretch. PKC epsilon expression was altered by cyclic stretch but not static stretch, while phospho PKC epsilon S719 remained unchanged. PKC alpha expression was not altered by stretch; however, phospho PKC alpha S657 increased in a dose-dependent manner following cyclic-perpendicular stretch. Our results indicate that changes in PKC expression and phosphorylation state may be a mechanism for cardiac myocytes to discriminate between the nature, direction, and intensity of mechanical stretch.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
PrkcaRatresponse to mechanical stimulus  IEP  RGD 
PrkcdRatresponse to mechanical stimulus  IEP  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Prkca  (protein kinase C, alpha)
Prkcd  (protein kinase C, delta)


Additional Information