RGD Reference Report - Isoform- and subcellular fraction-specific differences in hippocampal 14-3-3 levels following experimentally evoked seizures and in human temporal lobe epilepsy. - Rat Genome Database

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Isoform- and subcellular fraction-specific differences in hippocampal 14-3-3 levels following experimentally evoked seizures and in human temporal lobe epilepsy.

Authors: Schindler, CK  Heverin, M  Henshall, DC 
Citation: Schindler CK, etal., J Neurochem. 2006 Oct;99(2):561-9. Epub 2006 Sep 18.
RGD ID: 1625714
Pubmed: PMID:16981892   (View Abstract at PubMed)
DOI: DOI:10.1111/j.1471-4159.2006.04153.x   (Journal Full-text)

14-3-3 proteins are a family of signaling molecules involved in diverse cellular functions, which can mediate anti-apoptotic effects. Seizure-induced neuronal death may involve programmed (apoptotic) cell death pathways and is associated with a decline in brain 14-3-3 levels. Presently, we investigated the subcellular localization and effects of seizures on isoforms of 14-3-3 in rat hippocampus, and contrasted these to findings in human temporal lobe epilepsy (TLE). All brain isoforms of 14-3-3 were detected in the cytoplasmic compartment of rat hippocampus, while 14-3-3gamma and -zeta were also present in mitochondrial and microsome-enriched fractions. Focally evoked seizures in rats significantly reduced 14-3-3gamma levels within the microsome-enriched compartment at 4 h, with similar responses for 14-3-3zeta, while cytoplasm-localized 14-3-3beta, -epsilon and -eta remained unchanged. Analysis of human autopsy control hippocampus revealed similar 14-3-3 isoform expression profiles. In TLE samples, the microsome-enriched fraction also showed differences, but here 14-3-3epsilon and -zeta levels were higher than controls. TLE sample 14-3-3 isoform abundance within the cytoplasmic fraction was not different to controls. This study defines the subcellular localization of 14-3-3 isoforms in rat and human hippocampus and identifies the microsome-enriched fraction as the main site of altered 14-3-3 levels in response to acute prolonged and chronic recurrent seizures.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
YWHAZHumanExperimental Seizures  ISOYwhaz (Rattus norvegicus)protein:decreased expression:hippocampusRGD 
YwhazRatExperimental Seizures  IEP protein:decreased expression:hippocampusRGD 
YwhazMouseExperimental Seizures  ISOYwhaz (Rattus norvegicus)protein:decreased expression:hippocampusRGD 
YWHAQHumantemporal lobe epilepsy  IEP protein:decreased expression:mitochondria and hippocampusRGD 
YWHAZHumantemporal lobe epilepsy  IEP protein:increased expression:membrane-bounded organelle and hippocampusRGD 
YwhaqRattemporal lobe epilepsy  ISOYWHAQ (Homo sapiens)protein:decreased expression:mitochondria and hippocampusRGD 
YwhaqMousetemporal lobe epilepsy  ISOYWHAQ (Homo sapiens)protein:decreased expression:mitochondria and hippocampusRGD 
YwhazRattemporal lobe epilepsy  ISOYWHAZ (Homo sapiens)protein:increased expression:membrane-bounded organelle and hippocampusRGD 
YwhazMousetemporal lobe epilepsy  ISOYWHAZ (Homo sapiens)protein:increased expression:membrane-bounded organelle and hippocampusRGD 

Objects Annotated

Genes (Rattus norvegicus)
Ywhaq  (tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta)
Ywhaz  (tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta)

Genes (Mus musculus)
Ywhaq  (tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein theta)
Ywhaz  (tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide)

Genes (Homo sapiens)
YWHAQ  (tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein theta)
YWHAZ  (tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta)


Additional Information