RGD Reference Report - Postsynaptic alpha 4 beta 2 and alpha 7 type nicotinic acetylcholine receptors contribute to the local and endogenous acetylcholine-mediated synaptic transmissions in nigral dopaminergic neurons. - Rat Genome Database

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Postsynaptic alpha 4 beta 2 and alpha 7 type nicotinic acetylcholine receptors contribute to the local and endogenous acetylcholine-mediated synaptic transmissions in nigral dopaminergic neurons.

Authors: Matsubayashi, H  Amano, T  Seki, T  Sasa, M  Sakai, N 
Citation: Matsubayashi H, etal., Brain Res. 2004 Apr 16;1005(1-2):1-8.
RGD ID: 1600975
Pubmed: PMID:15044058   (View Abstract at PubMed)
DOI: DOI:10.1016/j.brainres.2004.01.040   (Journal Full-text)

The local and endogenous nicotinic neuronal transmissions of dopaminergic neurons in the substantia nigra were confirmed electrophysiologically using a slice-patch technique. After identifying dopaminergic neurons based on their electrophysiological characteristics, miniature postsynaptic inward currents were recorded in the presence of atropine (a muscarinic acetylcholine receptor antagonist), bicuculline (a GABA receptor antagonist) and L-glutamic acid diethyl ester (GDEE) (a non-selective glutamate receptor antagonist). Under conditions that eliminated muscarinic, GABAergic and glutamatergic synaptic transmissions, we found miniature currents that were inhibited by the specific neuronal nicotinic receptor antagonists, dihydro-beta-erythroidine (DHbetaE) and/or methyllycaconitine (MLA) (selective alpha4beta2 and/or alpha7 nicotinic acetylcholine receptor antagonists, respectively). Under the same extracellular conditions, local stimulations in the vicinity of a target neuron evoked excitatory postsynaptic inward currents (EPSCs). These EPSCs were elicited in an extracellular Ca(2+) dependent manner and were also blocked by DHbetaE and/or MLA. These results suggest that dopaminergic neurons in the substantia nigra receive excitatory cholinergic inputs that are mediated via at least two types of postsynaptic nicotinic receptors, namely alpha7 and alpha4beta2 subtypes.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Chrna7Ratneuronal action potential  IMP  RGD 
Chrna7Ratsynaptic transmission, cholinergic  IMP  RGD 

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Chrna4Ratcholinergic synapse is_active_inEXP PMID:15044058SynGO 
Chrna4Ratcholinergic synapse is_active_inIDA PMID:15044058SynGO 
Chrna7Ratcholinergic synapse is_active_inEXP PMID:15044058SynGO 
Chrna7Ratcholinergic synapse is_active_inIDA PMID:15044058SynGO 
Chrnb2Ratcholinergic synapse is_active_inEXP PMID:15044058SynGO 
Chrnb2Ratcholinergic synapse is_active_inIDA PMID:15044058SynGO 

Objects Annotated

Genes (Rattus norvegicus)
Chrna4  (cholinergic receptor nicotinic alpha 4 subunit)
Chrna7  (cholinergic receptor nicotinic alpha 7 subunit)
Chrnb2  (cholinergic receptor nicotinic beta 2 subunit)


Additional Information