RGD Reference Report - Developmental profiles of arylsulfatases A and B in rat cerebral cortex and spinal cord. - Rat Genome Database

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Developmental profiles of arylsulfatases A and B in rat cerebral cortex and spinal cord.

Authors: Van der Pal, RH  Klein, W  Van Golde, LM  Lopes-Cardozo, M 
Citation: van der Pal RH, etal., Biochim Biophys Acta. 1991 Feb 5;1081(3):315-20.
RGD ID: 1599226
Pubmed: PMID:1671824   (View Abstract at PubMed)

Arylsulfatases A (EC 3.1.6.1) and B (EC 3.1.6.12) are lysosomal enzymes that can remove sulfate groups from sulfatides and sulfo-glycosaminoglycans, respectively. The activities of these enzymes in cerebral cortex and in spinal cord of developing rat pups were measured. The tissues were homogenized and the arylsulfatases A and B in the soluble fraction were separated from each other by anion exchange chromatography on DE-52 cellulose. Subsequently, the enzyme activities were assayed with p-nitrocatechol sulfate as substrate at 37 degrees C and pH 5.6. We observed a developmental profile of arylsulfatase A, similar to that previously reported for cerebroside sulfatase (EC 3.1.6.8; (Van der Pal et al. (1990) Biochim. Biophys. Acta 1043, 91-96]. The activity of arylsulfatase A increased gradually during development, whereas arylsulfatase B rose more steeply, peaked around day 15 and declined thereafter. As a consequence the ratio between B and A forms of arylsulfatase dropped from about 4 in 1-week-old pups to 2.2 (cortex) and 0.7 (cord) in 7-week-old rat pups.



Objects referenced in this article
Gene Arsa arylsulfatase A Rattus norvegicus
Gene Arsb arylsulfatase B Rattus norvegicus

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