RGD Reference Report - Alleviation of brain injury by applying TGN-020 in the supraoptic nucleus via inhibiting vasopressin neurons in rats of focal ischemic stroke. - Rat Genome Database

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Alleviation of brain injury by applying TGN-020 in the supraoptic nucleus via inhibiting vasopressin neurons in rats of focal ischemic stroke.

Authors: Cui, Dan  Jia, Shuwei  Li, Tong  Li, Dongyang  Wang, Xiaoran  Liu, Xiaoyu  Wang, Yu-Feng 
Citation: Cui D, etal., Life Sci. 2021 Jan 1;264:118683. doi: 10.1016/j.lfs.2020.118683. Epub 2020 Oct 28.
RGD ID: 155646130
Pubmed: PMID:33127515   (View Abstract at PubMed)
DOI: DOI:10.1016/j.lfs.2020.118683   (Journal Full-text)


AIMS: To understand mechanisms underlying vasopressin hypersecretion in stroke and its association with brain injury, we investigated effects of blocking aquaporin 4 (AQP4) in the supraoptic nucleus (SON) on vasopressin neuronal activity and cerebral injuries in male rats of unilateral middle cerebral artery occlusion (MCAO).
MAIN METHODS: Establishing MCAO model without or with microinjection of TGN-020 into the SON, performing Western blots and immunohistochemistry and analyzing the expression levels and spatial distribution of functional proteins in the SON and/or the cerebral cortex.
KEY FINDINGS: MCAO increased plasma vasopressin levels, caused neurological damage and increased glycogen synthase kinase 3β (GSK-3β) in the SON and the cortex of MCAO side. In the SON, MCAO significantly increased c-Fos in vasopressin neurons and astrocytic somata in the ventral glial lamina. MCAO significantly reduced glial fibrillary acidic protein (GFAP) and AQP4 around vasopressin neurons, which accompanied separation of GFAP from AQP4. By contrast, blocking AQP4 by microinjection of TGN-020 into the SON blocked MCAO-evoked GSK-3β increase as well as the reduction of AQP4 relative to GFAP around vasopressin neurons in the SON. In the cortex, TGN-020 in the SON also blocked MCAO-evoked increase in GSK-3β while reduced neurological damages.
SIGNIFICANCE: These findings indicate that MCAO disrupts interactions of GFAP with AQP4 in astrocytic processes in the SON, which increases vasopressin neuronal activity. Blocking AQP4 in the SON can block abnormal activation of vasopressin neurons and alleviate ischemic brain injury, which provides novel targets for alleviating ischemic brain injury.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
AQP4Humanmiddle cerebral artery infarction treatmentISOAqp4 (Rattus norvegicus) RGD 
Aqp4Ratmiddle cerebral artery infarction treatmentIMP  RGD 
Aqp4Mousemiddle cerebral artery infarction treatmentISOAqp4 (Rattus norvegicus) RGD 

Objects Annotated

Genes (Rattus norvegicus)
Aqp4  (aquaporin 4)

Genes (Mus musculus)
Aqp4  (aquaporin 4)

Genes (Homo sapiens)
AQP4  (aquaporin 4)


Additional Information