RGD Reference Report - QTL mapping for traits associated with stress neuroendocrine reactivity in rats. - Rat Genome Database

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QTL mapping for traits associated with stress neuroendocrine reactivity in rats.

Authors: Llamas, B  Contesse, V  Guyonnet-Duperat, V  Vaudry, H  Mormede, P  Moisan, MP 
Citation: Llamas B, etal., Mamm Genome 2005 Jul;16(7):505-15.
RGD ID: 1549862
Pubmed: (View Article at PubMed) PMID:16151695
DOI: Full-text: DOI:10.1007/s00335-005-0022-2

In the present study we searched for quantitative trait loci (QTLs) that affect neuroendocrine stress responses in a 20-min restraint stress paradigm using Brown-Norway (BN) and Wistar-Kyoto-Hyperactive (WKHA) rats. These strains differed in their hypothalamic-pituitary-adrenal axis (plasma ACTH and corticosterone levels, thymus, and adrenal weights) and in their renin-angiotensin-aldosterone system reactivity (plasma renin activity, aldosterone concentration). We performed a whole-genome scan on a F(2) progeny derived from a WKHA x BN intercross, which led to the identification of several QTLs linked to plasma renin activity (Sr6, Sr8, Sr11, and Sr12 on chromosomes RNO2, 3, 19, and 8, respectively), plasma aldosterone concentration (Sr7 and Sr9 on RNO2 and 5, respectively), and thymus weight (Sr10, Sr13, and Srl4 on RNO5, 10, and 16, respectively). The type 1b angiotensin II receptor gene (Agtrlb) maps within the confidence intervals of QTLs on RNO2 linked to plasma renin activity (Sr6, highly significant; LOD = 5.0) and to plasma aldosterone level (Sr7, suggestive; LOD = 2.0). In vitro studies of angiotensin II-induced release of aldosterone by adrenal glomerulosa cells revealed a lower receptor potency (log EC(50) = -8.16 +/- 0.11 M) and efficiency (E(max) = 453.3 +/- 25.9 pg/3 x 10(4) cells/24 h) in BN than in WKHA (log EC(50) = -10.66 +/- 0.18 M; E(max) = 573.1 +/- 15.3 pg/3 x 10(4) cells/24 h). Moreover, differences in Agtr1b mRNA abundance and sequence reinforce the putative role of the Agtr1b gene in the differential plasma renin stress reactivity between the two rat strains.

Annotation

Disease Annotations    

Objects Annotated

QTLs
Stresp14  (Stress response QTL 14)
Stresp15  (Stress response QTL 15)
Stresp16  (Stress response QTL 16)
Stresp17  (Stress response QTL 17)
Stresp18  (Stress response QTL 18)
Stresp19  (Stress response QTL 19)
Stresp20  (Stress response QTL 20)
Stresp21  (Stress response QTL 21)
Stresp22  (Stress response QTL 22)

Objects referenced in this article
Strain WKHA/Bord null Rattus norvegicus

Additional Information