RGD Reference Report - Clinical and biological significance of miR-23b and miR-193a in human hepatocellular carcinoma. - Rat Genome Database

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Clinical and biological significance of miR-23b and miR-193a in human hepatocellular carcinoma.

Authors: Grossi, Ilaria  Arici, Bruna  Portolani, Nazario  De Petro, Giuseppina  Salvi, Alessandro 
Citation: Grossi I, etal., Oncotarget. 2017 Jan 24;8(4):6955-6969. doi: 10.18632/oncotarget.14332.
RGD ID: 153344564
Pubmed: PMID:28036298   (View Abstract at PubMed)
PMCID: PMC5351682   (View Article at PubMed Central)
DOI: DOI:10.18632/oncotarget.14332   (Journal Full-text)

Hepatocellular carcinoma (HCC) is the most common cancer of the liver with a very poor prognosis. The dysregulation of microRNAs (miRs) is indeed implicated in HCC onset and progression. In this study, we have evaluated the expression of miR-23b and miR-193a in a large cohort of 59 and 67 HCC patients, respectively. miR-23b and miR-193a resulted significantly down-regulated in primary HCCs compared to their matched peritumoral counterparts. Furthermore, patients with higher miR-193a expression exhibited longer OS and DFS, suggesting that miR-193a may be a molecular prognostic factor for HCC patients. Since the regulation of miRs by DNA methylation may occur in human cancers, we verified whether the down-modulation of miR-23b and miR-193a in HCC tissues could be related to DNA methylation. An inverse trend between miR-23b expression and DNA methylation was observed, indicating that miR-23b can be epigenetically regulated. By contrast, the down-regulation of miR-193a was not mediated by DNA methylation. To verify the potential role of miR-23b and miR-193a as responsive molecular targets in vitro, we used the inhibitor of DNA methylation 5-aza-dC to restore miR-23b expression level in combination with miR-193a transfection. The combined treatment led to a significant inhibition of cellular proliferation and migration. Taken together, our findings provide evidence that miR-23b and miR-193a may be molecular diagnostic and prognostic factors for HCC; furthermore, miR-23b and miR-193a are responsive molecular targets for limiting HCC cell aggressiveness in combination with the epigenetic drug 5-aza-dC. Moreover, our results provide new advances in the epigenetic regulation of these miRs in HCC.




  
Object Symbol
Species
Term
Qualifier
Evidence
With
Notes
Source
Original Reference(s)
MIR193AHumanhepatocellular carcinoma  IDA DNA:hypomethylationRGD 
MIR23BHumanhepatocellular carcinoma  IDA DNA:hypermethylationRGD 
Mir193aRathepatocellular carcinoma  ISOMIR193A (Homo sapiens)DNA:hypomethylationRGD 
Mir193aMousehepatocellular carcinoma  ISOMIR193A (Homo sapiens)DNA:hypomethylationRGD 
Mir23bMousehepatocellular carcinoma  ISOMIR23B (Homo sapiens)DNA:hypermethylationRGD 
Mir23bRathepatocellular carcinoma  ISOMIR23B (Homo sapiens)DNA:hypermethylationRGD 


  
Object Symbol
Species
Term
Qualifier
Evidence
With
Notes
Source
Original Reference(s)
MIR193AHuman5-azacytidine increases expression EXP 5-azacytidine increases expression of MIR193A in liver cellsRGD 
MIR23BHuman5-azacytidine increases expressionEXP 5-azacytidine increases expression of MIR23B miRNA in liver cellsRGD 
Mir193aMouse5-azacytidine increases expression ISOMIR193A (Homo sapiens)5-azacytidine increases expression of MIR193A in liver cellsRGD 
Mir193aRat5-azacytidine increases expression ISOMIR193A (Homo sapiens)5-azacytidine increases expression of MIR193A in liver cellsRGD 
Mir23bMouse5-azacytidine increases expressionISOMIR23B (Homo sapiens)5-azacytidine increases expression of MIR23B miRNA in liver cellsRGD 
Mir23bRat5-azacytidine increases expressionISOMIR23B (Homo sapiens)5-azacytidine increases expression of MIR23B miRNA in liver cellsRGD 


Genes (Rattus norvegicus)
Mir193a  (microRNA 193a) Mir23b  (microRNA 23b)

Genes (Mus musculus)
Mir193a  (microRNA 193a) Mir23b  (microRNA 23b)

Genes (Homo sapiens)
MIR193A  (microRNA 193a) MIR23B  (microRNA 23b)