RGD Reference Report - The synthetic flavonoid WYC02-9 inhibits colorectal cancer cell growth through ROS-mediated activation of MAPK14 pathway. - Rat Genome Database

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The synthetic flavonoid WYC02-9 inhibits colorectal cancer cell growth through ROS-mediated activation of MAPK14 pathway.

Authors: Chen, Yun-Ju  Chen, Hsin-Pao  Cheng, Yu-Jen  Lin, Yu-Heng  Liu, Kuang-Wen  Chen, Yun-Ju  Hou, Ming-Feng  Wu, Yang-Chang  Lee, Yi-Chen  Yuan, Shyng-Shiou 
Citation: Chen YJ, etal., Life Sci. 2013 Jun 13;92(22):1081-92. doi: 10.1016/j.lfs.2013.04.007. Epub 2013 Apr 26.
RGD ID: 151665352
Pubmed: PMID:23624232   (View Abstract at PubMed)
DOI: DOI:10.1016/j.lfs.2013.04.007   (Journal Full-text)


AIM: Colorectal cancer (CRC) is a leading cause of cancer-related deaths worldwide. In this study, we explored the anti-cancer activity of WYC02-9, a synthetic protoapigenone, on human HCT116 CRC cells.
MAIN METHODS: The anti-cancer activity of WYC02-9 and its underlying mechanisms were analyzed using XTT cell proliferation assays, colony formation assays, FACS analysis, annexin V staining, immunoblotting analysis, reactive oxygen species (ROS) generation assays, soft agar assays, a nude mice xenograft study and immunohistochemistry assays.
KEY FINDINGS: Data showed that WYC02-9 suppressed CRC cell growth by arresting cells at G2/M and inducing cell death via apoptotic pathways. Further analysis demonstrated that WYC02-9-induced apoptosis was mediated by the activation of a ROS-mediated MAPK14 pathway. An in vivo xenograft study revealed that WYC02-9 enhanced MAP2K3/6 and MAPK14 phosphorylation, induced apoptosis, and suppressed CRC tumor growth.
SIGNIFICANCE: WYC02-9 exerts its anti-tumor effect via ROS/MAPK14-induced apoptosis and has the potential to be developed as a chemotherapeutic agent for CRC.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
MAPK14Humancolorectal carcinoma treatmentIDA  RGD 
Mapk14Ratcolorectal carcinoma treatmentISOMAPK14 (Homo sapiens) RGD 
Mapk14Mousecolorectal carcinoma treatmentISOMAPK14 (Homo sapiens) RGD 

Objects Annotated

Genes (Rattus norvegicus)
Mapk14  (mitogen activated protein kinase 14)

Genes (Mus musculus)
Mapk14  (mitogen-activated protein kinase 14)

Genes (Homo sapiens)
MAPK14  (mitogen-activated protein kinase 14)


Additional Information