RGD Reference Report - Measurement of Autoantibodies to Gastric H+,K+-ATPase (ATP4A/B) Using a Luciferase Immunoprecipitation System (LIPS). - Rat Genome Database

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Measurement of Autoantibodies to Gastric H+,K+-ATPase (ATP4A/B) Using a Luciferase Immunoprecipitation System (LIPS).

Authors: Lahner, Edith  Marzinotto, Ilaria  Brigatti, Cristina  Davidson, Howard  Wenzlau, Janet  Piemonti, Lorenzo  Annibale, Bruno  Lampasona, Vito 
Citation: Lahner E, etal., Methods Mol Biol. 2019;1901:113-131. doi: 10.1007/978-1-4939-8949-2_10.
RGD ID: 14696739
Pubmed: PMID:30539573   (View Abstract at PubMed)
DOI: DOI:10.1007/978-1-4939-8949-2_10   (Journal Full-text)

The Luciferase Immuno Precipitation System (LIPS) enables the detection of specific serum antibodies by immunoprecipitation of recombinant antigens tagged with a luciferase reporter. Here we describe LIPS assays for the quantification of autoantibodies to the H+, K+-ATPase A (ATP4A) and B (ATP4B) subunits, two serological markers of autoimmune atrophic gastritis and pernicious anemia. In particular, we will describe the expression of luciferase-tagged recombinant ATP4A and ATP4B, their immunoprecipitation with test sera, the recovery and washing of immune-complexes with a protein-A coated resin, and the quantification of autoantibodies by addition of a luciferase substrate and the measurement of the light output from captured luciferase-tagged antigens.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
ATP4BHumanatrophic gastritis  IDA  RGD 
Atp4bRatatrophic gastritis  ISOATP4B (Homo sapiens) RGD 
Atp4bMouseatrophic gastritis  ISOATP4B (Homo sapiens) RGD 

Objects Annotated

Genes (Rattus norvegicus)
Atp4b  (ATPase H+/K+ transporting subunit beta)

Genes (Mus musculus)
Atp4b  (ATPase, H+/K+ exchanging, beta polypeptide)

Genes (Homo sapiens)
ATP4B  (ATPase H+/K+ transporting subunit beta)


Additional Information