RGD Reference Report - Knockdown of E2F3 Inhibits Proliferation, Migration, and Invasion and Increases Apoptosis in Glioma Cells. - Rat Genome Database

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Knockdown of E2F3 Inhibits Proliferation, Migration, and Invasion and Increases Apoptosis in Glioma Cells.

Authors: Shen, Zhi-Gang  Liu, Xiao-Zhou  Chen, Chang-Xiu  Lu, Jing-Min 
Citation: Shen ZG, etal., Oncol Res. 2017 Nov 2;25(9):1555-1566. doi: 10.3727/096504017X14897158009178. Epub 2017 Mar 23.
RGD ID: 13702470
Pubmed: PMID:28337965   (View Abstract at PubMed)
PMCID: PMC7841128   (View Article at PubMed Central)
DOI: DOI:10.3727/096504017X14897158009178   (Journal Full-text)

E2F3a, as a member of the E2F family, is essential for cell division associated with the progression of many cancers. However, the biological effect of E2F3a on glioma is not understood as well. To investigate the functional mechanism of E2F3a in glioma, we examined the expression of E2F3a in glioma tissue and cell lines. We found that E2F3a was upregulated in glioma tissue compared with adjacent tissue, and this was associated with a poor survival rate. E2F3a was highly expressed in glioma cell lines compared with normal HEB cell lines. Knockdown of E2F3a significantly inhibited cell proliferation, promoted G0/G1 phase arrest, elevated apoptosis rates, and suppressed cell migration and invasion. However, overexpression of E2F3a markedly promoted cell proliferation, migration, and invasion and inhibited apoptosis. Moreover, in vivo studies showed that knockdown of E2F3a expression dramatically inhibited U373 tumor growth in a nude mouse model. Results of real-time PCR and Western blot showed that the depletion of E2F3a upregulated the expression levels of cell apoptosis-related proteins and downregulated migration-related proteins. Conversely, E2F3a overexpression downregulated the expression levels of cell apoptosis-related proteins and upregulated migration-related proteins. In conclusion, our results highlight the importance of E2F3a in glioma and provide new insights into the diagnostics and therapeutics of gliomas.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
E2F3Humanhigh grade glioma disease_progressionIEP  RGD 
E2f3Mousehigh grade glioma disease_progressionISOE2F3 (Homo sapiens) RGD 
E2f3Rathigh grade glioma disease_progressionISOE2F3 (Homo sapiens) RGD 

Objects Annotated

Genes (Rattus norvegicus)
E2f3  (E2F transcription factor 3)

Genes (Mus musculus)
E2f3  (E2F transcription factor 3)

Genes (Homo sapiens)
E2F3  (E2F transcription factor 3)


Additional Information