RGD Reference Report - Distribution of soluble N-ethylmaleimide fusion protein attachment proteins (SNAPs) in the rat nervous system. - Rat Genome Database

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Distribution of soluble N-ethylmaleimide fusion protein attachment proteins (SNAPs) in the rat nervous system.

Authors: Nishiki, T  Nihonmatsu, I  Tsuhara, Y  Kawasaki, M  Sekiguchi, M  Sato, K  Mizoguchi, A  Takahashi, M 
Citation: Nishiki T, etal., Neuroscience. 2001;107(3):363-71.
RGD ID: 13702225
Pubmed: PMID:11718992   (View Abstract at PubMed)

Soluble N-ethylmaleimide-sensitive fusion protein (NSF) attachment protein (SNAP) plays an essential role in vesicular transport and the release of neurotransmitters and hormones through associations with NSF and SNAP receptors (SNAREs). Three isoforms (alpha, beta and gamma) of SNAP are expressed in mammals. We have generated isoform-specific antibodies and studied the expression and distribution of these SNAP isoforms in the rat nervous system. Each antibody specifically recognized alpha-, beta- or gamma-SNAP in an isoform-specific manner in immunoblots of brain homogenate. Alpha- and gamma-SNAP were ubiquitously expressed in various tissues, whereas beta-SNAP was expressed only in brain. After subcellular fractionation of brain homogenates, all three isoforms were recovered in both soluble and particulate fractions. Immunohistochemistry revealed that alpha- and beta-SNAP were generally differentially distributed both in synaptic and non-synaptic regions, including brain white matter. The presynaptic location of both alpha- and beta-SNAP was confirmed by immunoelectron microscopy. At the neuromuscular junction, immunoreactive alpha-SNAP was identified in synaptic vesicles, while in the cerebellum, beta-SNAP was present in the presynaptic membranes of basket neuron and mossy fiber terminals. From these results we suggest that both alpha- and beta-SNAP may play an important role in neurotransmitter release as well as in constitutive vesicular transport.



Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
NapaRatneuromuscular junction is_active_inIDA PMID:11718992SynGO 
NapaRatpostsynapse is_active_inIDA PMID:11718992SynGO 
NapaRatpostsynapse is_active_inIEP PMID:11718992SynGO 
NapbRatpostsynapse is_active_inIEP PMID:11718992SynGO 
NapbRatpostsynapse is_active_inIDA PMID:11718992SynGO 
NapaRatpresynapse is_active_inIDA PMID:11718992SynGO 
NapaRatpresynapse is_active_inIEP PMID:11718992SynGO 
NapbRatpresynapse is_active_inIEP PMID:11718992SynGO 
NapbRatpresynapse is_active_inIDA PMID:11718992SynGO 
NapaRatpresynaptic active zone membrane is_active_inIDA PMID:11718992SynGO 
NapbRatpresynaptic active zone membrane is_active_inIDA PMID:11718992SynGO 
NapgRatsynapse is_active_inIDA PMID:11718992SynGO 
NapgRatsynapse is_active_inIEP PMID:11718992SynGO 

Objects Annotated

Genes (Rattus norvegicus)
Napa  (NSF attachment protein alpha)
Napb  (NSF attachment protein beta)
Napg  (NSF attachment protein gamma)


Additional Information