RGD Reference Report - Up-regulation of P2X2, P2X4 receptor and ischemic cell death: prevention by P2 antagonists. - Rat Genome Database

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Up-regulation of P2X2, P2X4 receptor and ischemic cell death: prevention by P2 antagonists.

Authors: Cavaliere, F  Florenzano, F  Amadio, S  Fusco, F R  Viscomi, M T  D'Ambrosi, N  Vacca, F  Sancesario, G  Bernardi, G  Molinari, M  Volontè, C 
Citation: Cavaliere F, etal., Neuroscience. 2003;120(1):85-98.
RGD ID: 13673769
Pubmed: PMID:12849743   (View Abstract at PubMed)

In the present work we examined the involvement of selected P2X receptors for extracellular ATP in the onset of neuronal cell death caused by glucose/oxygen deprivation. The in vitro studies of organotypic cultures from hippocampus evidenced that P2X2 and P2X4 were up-regulated by glucose/oxygen deprivation. Moreover, we showed that ischemic conditions induced specific neuronal loss not only in hippocampal, but also in cortical and striatal organotypic cultures and the P2 receptor antagonists basilen blue and suramin prevented these detrimental effects. In the in vivo experiments we confirmed the induction of P2X receptors in the hippocampus of gerbils subjected to bilateral common carotid occlusion. In particular, P2X2 and P2X4 proteins became significantly up-regulated, although to different extent and in different cellular phenotypes. The induction was confined to the pyramidal cell layer of the CA1 subfield and to the transition zone of the CA2 subfield and it was coincident with the area of neuronal damage. P2X2 was expressed in neuronal cell bodies and fibers in the CA1 pyramidal cell layer and in the strata oriens and radiatum. Intense P2X4 immunofluorescence was localized to microglia cells. Our results indicate a direct involvement of P2X receptors in the mechanisms sustaining cell death evoked by metabolism impairment and suggest the use of selected P2 antagonists as effective neuroprotecting agents.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
P2rx2Ratresponse to ischemia involved_inIMP PMID:12849743ARUK-UCL 
P2rx4Ratresponse to ischemia involved_inIMP PMID:12849743ARUK-UCL 

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
P2rx4Ratcell body located_inIDA PMID:12849743ARUK-UCL 
P2rx2Ratneuronal cell body located_inIDA PMID:12849743ARUK-UCL 

Objects Annotated

Genes (Rattus norvegicus)
P2rx2  (purinergic receptor P2X 2)
P2rx4  (purinergic receptor P2X 4)


Additional Information