RGD Reference Report - [Expression and methylation of adenomatous polyposis coli gene in endometrioid adenocarcinoma]. - Rat Genome Database

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[Expression and methylation of adenomatous polyposis coli gene in endometrioid adenocarcinoma].

Authors: Qian, Bing  Ke, Pei-Qi  Wang, Li  Liu, Wen-Ji  Li, Mei-Xiang 
Citation: Qian B, etal., Ai Zheng. 2008 Jun;27(6):585-9.
RGD ID: 13432144
Pubmed: (View Article at PubMed) PMID:18570730


BACKGROUND & OBJECTIVE: Endometrial carcinoma is a common malignant tumor of female reproductive system, with an increasing incidence in China. Adenomatous polyposis coli (APC) gene, a tumor suppressor gene, is expressed in many tissues, and has a certain relationship with ovarian cancer. This study was to observe the expression and DNA methylation of APC gene in endometrioid adenocarcinoma, and explore its correlations to the occurrence and development of this disease.
METHODS: The methylation, mRNA and protein expression of APC gene were detected by methylation-specific polymerase chain reaction (PCR), reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry in 30 specimens of normal proliferative endometrium, 30 specimens of atypical hyperplastic endometrium and 60 specimens of endometrioid adenocarcinoma.
RESULTS: The methylation rate of APC gene was significantly higher, the positive rates of APC mRNA and protein were significantly lower in endometrioid adenocarcinoma than in atypical hyperplastic endometrium and normal proliferative endometrium (65.0% vs. 33.3% and 23.3%, 33.3% vs. 63.3% and 73.3%, 30.0% vs. 50.0% and 66.7%,P<0.05). There was no significant difference between atypical hyperplastic endometrium and normal endometrium (P>0.05). APC methylation was positively correlated to APC mRNA expression.
CONCLUSION: The expression and DNA methylation of APC gene are certainly related with the occurrence and development of endometrioid adenocarcinoma.

Annotation

Disease Annotations    
Endometrioid Carcinomas  (IAGP,IEP,ISO)

Objects Annotated

Genes (Rattus norvegicus)
Apc  (APC regulator of WNT signaling pathway)

Genes (Mus musculus)
Apc  (APC, WNT signaling pathway regulator)

Genes (Homo sapiens)
APC  (APC regulator of WNT signaling pathway)


Additional Information