RGD Reference Report - Localization of the Cl(-)-ATPase activity on NAP-22 enriched membrane microdomain (raft) of rat brain. - Rat Genome Database

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Localization of the Cl(-)-ATPase activity on NAP-22 enriched membrane microdomain (raft) of rat brain.

Authors: Maekawa, S  Taguchi, K 
Citation: Maekawa S and Taguchi K, Neurosci Lett 2004 May 20;362(2):158-61.
RGD ID: 1304336
Pubmed: PMID:15193776   (View Abstract at PubMed)
DOI: DOI:10.1016/j.neulet.2004.03.020   (Journal Full-text)

Much attention has been paid to the membrane microdomain enriched in cholesterol and sphingolipids called raft. In the central nervous system, however, the physiological role of this domain is not so evident at present, partly because of the complexity of the protein components in the raft fraction. In this study we surveyed ATPase activities in the raft fraction obtained from the synaptic plasma membrane of rat brain and found the enrichment of an ethacrynic acid-sensitive ATPase (Cl(-)-pump) activity. Immunoprecipitation experiments using antibodies to raft-localized proteins showed the co-precipitation of the ATPase activity with NAP-22, a major raft-localized protein. This result suggests the participation of the raft in the regulation of ion transport in addition to the presence of heterogeneity of raft domains in neurons.

Objects referenced in this article
Gene Basp1 brain abundant, membrane attached signal protein 1 Rattus norvegicus

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