RGD Reference Report - Combining congenic coverage with gene profiling in search of candidates for blood pressure quantitative trait loci in Dahl rats. - Rat Genome Database

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Combining congenic coverage with gene profiling in search of candidates for blood pressure quantitative trait loci in Dahl rats.

Authors: Moujahidine, M  Lambert, R  Dutil, J  Palijan, A  Sivo, Z  Ariyarajah, A  Deng, AY 
Citation: Moujahidine M, etal., Hypertens Res 2004 Mar;27(3):203-12.
RGD ID: 1300037
Pubmed: PMID:15080379   (View Abstract at PubMed)

Chromosomes (Chr) 10 and 16 of the Dahl salt-sensitive (S) rat harbor quantitative trait loci (QTLs) for blood pressure (BP). To facilitate gene discovery of these QTLs, gene profiling based on microarrays was combined with fine QTL mapping to identify potential candidate genes that are differentially expressed. First, the region harboring the BP QTL on Chr 16 was narrowed by comparative congenic mapping. In this endeavor, a number of new chromosome markers were generated and used to physically define the chromosome interval in question. Second, in an effort to minimize the costs of gene profiling without sacrificing the chance of gene discovery, a combination congenic strain was produced by replacing one segment of Chr 10 along with one segment of Chr 16 of the hypertensive S rat by those of the normotensive Lewis (LEW) rat. Both of these regions are known to contain BP QTLs. Third, kidneys of this combination congenic strain and the S strain were employed for expression profiling studies. Finally, a comparison between the two strains yielded a number of potentially differentially expressed candidates. Six Established Sequence Tags (ESTs)/genes among them were located in Chr 10 regions and 1 was found in a Chr 16 region, and the genetic make-ups of all these regions were shown to be different between S and LEW. However, none of these ESTs/genes identified by gene profiling were located in an interval containing a QTL. Thus, the present study highlights the importance of correlating the results of gene expression profiling with fine congenic mapping.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Bp248Rathypertension  IAGP  RGD 

Phenotype Annotations    Click to see Annotation Detail View

Mammalian Phenotype

Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Bp248Ratincreased systemic arterial blood pressure  IAGP  RGD 

Related Phenotype Data for this Reference

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Objects Annotated

QTLs
Bp248  (Blood pressure QTL 248)

Objects referenced in this article
Marker D16Chm68 D16Chm68 Rattus norvegicus
Marker D16Chm65 D16Chm65 Rattus norvegicus
Marker D16Chm52 D16Chm52 Rattus norvegicus
Marker D16Chm14 D16Chm14 Rattus norvegicus
Marker D16Chm71 D16Chm71 Rattus norvegicus
Marker D16Chm73 D16Chm73 Rattus norvegicus
Marker D16Chm61 D16Chm61 Rattus norvegicus
Marker D16Chm49 D16Chm49 Rattus norvegicus
Marker D16Chm27 D16Chm27 Rattus norvegicus
Marker D16Chm16 D16Chm16 Rattus norvegicus
Marker D16Chm78 D16Chm78 Rattus norvegicus
Marker D16Chm53 D16Chm53 Rattus norvegicus
Marker D16Chm26 D16Chm26 Rattus norvegicus
Marker D16Chm43 D16Chm43 Rattus norvegicus
Marker D16Chm32 D16Chm32 Rattus norvegicus
Marker D16Chm34 D16Chm34 Rattus norvegicus
Marker D16Chm42 D16Chm42 Rattus norvegicus
Marker D16Chm67 D16Chm67 Rattus norvegicus
Marker D16Chm64 D16Chm64 Rattus norvegicus
Marker D16Chm31 D16Chm31 Rattus norvegicus
Marker D16Chm58 D16Chm58 Rattus norvegicus
Marker D16Chm33 D16Chm33 Rattus norvegicus
Marker D16Chm77 D16Chm77 Rattus norvegicus
Marker D16Chm74 D16Chm74 Rattus norvegicus
Marker D16Chm25 D16Chm25 Rattus norvegicus
Marker D16Chm55 D16Chm55 Rattus norvegicus
Marker D16Chm69 D16Chm69 Rattus norvegicus
Marker D16Chm24 D16Chm24 Rattus norvegicus
Marker D16Chm63 D16Chm63 Rattus norvegicus
Marker D16Chm47 D16Chm47 Rattus norvegicus
Marker D16Chm23 D16Chm23 Rattus norvegicus
Marker D16Chm6 D16Chm6 Rattus norvegicus
Marker D16Chm12 D16Chm12 Rattus norvegicus
Marker D16Chm36 D16Chm36 Rattus norvegicus
Marker D16Chm60 D16Chm60 Rattus norvegicus
Marker D16Chm66 D16Chm66 Rattus norvegicus
Marker D16Chm29 D16Chm29 Rattus norvegicus
Marker D16Chm2 D16Chm2 Rattus norvegicus
Marker D16Chm54 D16Chm54 Rattus norvegicus
Marker D16Chm76 D16Chm76 Rattus norvegicus
Marker D16Chm41 D16Chm41 Rattus norvegicus
Marker D16Chm37 D16Chm37 Rattus norvegicus
Marker D16Chm48 D16Chm48 Rattus norvegicus
Marker D16Chm7 D16Chm7 Rattus norvegicus
Marker D16Chm46 D16Chm46 Rattus norvegicus
QTL Bp75 Blood pressure QTL 75 Rattus norvegicus
Strain SS.LEW-(D10Rat119-D10Mgh1)(D16Rat21-D16Rat112)/Ayd null Rattus norvegicus
Strain SS.LEW-(D16Mit3-D16Rat112)/Ayd null Rattus norvegicus
Strain SS.LEW-(D16Rat12-D16Chm23)/Ayd null Rattus norvegicus
Strain SS.LEW-(D16Rat38-D16Chm66)/Ayd null Rattus norvegicus

Additional Information