RGD Reference Report - Hepatitis C Virus-Induced Upregulation of MicroRNA miR-146a-5p in Hepatocytes Promotes Viral Infection and Deregulates Metabolic Pathways Associated with Liver Disease Pathogenesis. - Rat Genome Database

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Hepatitis C Virus-Induced Upregulation of MicroRNA miR-146a-5p in Hepatocytes Promotes Viral Infection and Deregulates Metabolic Pathways Associated with Liver Disease Pathogenesis.

Authors: Bandiera, Simonetta  Pernot, Sophie  El Saghire, Hussein  Durand, Sarah C  Thumann, Christine  Crouchet, Emilie  Ye, Tao  Fofana, Isabel  Oudot, Marine A  Barths, Jochen  Schuster, Catherine  Pessaux, Patrick  Heim, Markus H  Baumert, Thomas F  Zeisel, Mirjam B 
Citation: Bandiera S, etal., J Virol. 2016 Jun 24;90(14):6387-6400. doi: 10.1128/JVI.00619-16. Print 2016 Jul 15.
RGD ID: 126925147
Pubmed: PMID:27147737   (View Abstract at PubMed)
PMCID: PMC4936128   (View Article at PubMed Central)
DOI: DOI:10.1128/JVI.00619-16   (Journal Full-text)


UNLABELLED: Hepatitis C virus (HCV)-induced chronic liver disease is a leading cause of hepatocellular carcinoma (HCC). However, the molecular mechanisms underlying HCC development following chronic HCV infection remain poorly understood. MicroRNAs (miRNAs) play an important role in homeostasis within the liver, and deregulation of miRNAs has been associated with liver disease, including HCC. While host miRNAs are essential for HCV replication, viral infection in turn appears to induce alterations of intrahepatic miRNA networks. Although the cross talk between HCV and liver cell miRNAs most likely contributes to liver disease pathogenesis, the functional involvement of miRNAs in HCV-driven hepatocyte injury and HCC remains elusive. Here we combined a hepatocyte-like cell-based model system, high-throughput small RNA sequencing, computational analysis, and functional studies to investigate HCV-miRNA interactions that may contribute to liver disease and HCC. Profiling analyses indicated that HCV infection differentially regulated the expression of 72 miRNAs by at least 2-fold, including miRNAs that were previously described to target genes associated with inflammation, fibrosis, and cancer development. Further investigation demonstrated that the miR-146a-5p level was consistently increased in HCV-infected hepatocyte-like cells and primary human hepatocytes, as well as in liver tissue from HCV-infected patients. Genome-wide microarray and computational analyses indicated that miR-146a-5p overexpression modulates pathways that are related to liver disease and HCC development. Furthermore, we showed that miR-146a-5p has a positive impact on late steps of the viral replication cycle, thereby increasing HCV infection. Collectively, our data indicate that the HCV-induced increase in miR-146a-5p expression both promotes viral infection and is relevant for pathogenesis of liver disease.
IMPORTANCE: HCV is a leading cause of chronic liver disease and cancer. However, how HCV induces liver cancer remains poorly understood. There is accumulating evidence that a viral cure does not eliminate the risk for HCC development. Thus, there is an unmet medical need to develop novel approaches to predict and prevent virus-induced HCC. miRNA expression is known to be deregulated in liver disease and cancer. Furthermore, miRNAs are essential for HCV replication, and HCV infection alters miRNA expression. However, how miRNAs contribute to HCV-driven pathogenesis remains elusive. Here we show that HCV induces miRNAs that may contribute to liver injury and carcinogenesis. The miR-146a-5p level was consistently increased in different cell-based models of HCV infection and in HCV patient-derived liver tissue. Furthermore, miR-146a-5p increased HCV infection. Collectively, our data are relevant to understanding viral pathogenesis and may open perspectives for novel biomarkers and prevention of virus-induced liver disease and HCC.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
MIR146AHumanChronic Hepatitis C  IEP miRNA:increased expression:liver (human)RGD 
Mir146MouseChronic Hepatitis C  ISOMIR146A (Homo sapiens)miRNA:increased expression:liver (human)RGD 
Mir146aRatChronic Hepatitis C  ISOMIR146A (Homo sapiens)miRNA:increased expression:liver (human)RGD 

Objects Annotated

Genes (Rattus norvegicus)
Mir146a  (microRNA 146a)

Genes (Mus musculus)
Mir146  (microRNA 146)

Genes (Homo sapiens)
MIR146A  (microRNA 146a)


Additional Information