RGD Reference Report - Interleukin-6 receptor blockade suppresses subretinal fibrosis in a mouse model. - Rat Genome Database

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Interleukin-6 receptor blockade suppresses subretinal fibrosis in a mouse model.

Authors: Cui, W  Zhang, H  Liu, ZL 
Citation: Cui W, etal., Int J Ophthalmol. 2014 Apr 18;7(2):194-7. doi: 10.3980/j.issn.2222-3959.2014.02.02. eCollection 2014.
RGD ID: 10402815
Pubmed: PMID:24790857   (View Abstract at PubMed)
PMCID: PMC4003069   (View Article at PubMed Central)
DOI: DOI:10.3980/j.issn.2222-3959.2014.02.02   (Journal Full-text)

AIM: To determine the involvement of the interleukin (IL)-6 with the development of experimental subretinal fibrosis in a mouse model. METHODS: Subretinal fibrosis was induced by subretinal injection of macrophage-rich peritoneal exudate cells and the local expression of IL-6 was assessed by quantitative real-time reverse transcription-polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA) at various time points. In addition, we investigated the effect of IL-6 receptor (IL-6R) monoclonal antibody (MR16-1) on subretinal fibrosis development. RESULTS: IL-6 mRNA level was significantly elevated at 1d after subretinal fibrosis induction and increased further to about 12-fold at 2d, reaching the peak. The result of ELISA showed that IL-6 protein was not detected in naive mice. At 2d after subretinal fibrosis induction, IL-6 protein level was upregulated to 67.33+/-14.96 pg/mg in subretinal fibrosis mice. MR16-1 treatment resulted in a reduced subretinal fibrosis area by 48% compared to animals from control group at 7d. CONCLUSION: Our results indicated that IL-6 signaling may contribute to the pathogenesis of subretinal fibrogenesis and IL-6R inhibition may provide an effective, novel treatment of advanced and late-stage neovascular age-related macular degeneration.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
IL6HumanSubretinal Fibrosis  ISOIl6 (Mus musculus) RGD 
IL6RHumanSubretinal Fibrosis treatmentISOIl6ra (Mus musculus) RGD 
Il6RatSubretinal Fibrosis  ISOIl6 (Mus musculus) RGD 
Il6MouseSubretinal Fibrosis  IEP  RGD 
Il6rRatSubretinal Fibrosis treatmentISOIl6ra (Mus musculus) RGD 
Il6raMouseSubretinal Fibrosis treatmentIMP  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Il6  (interleukin 6)
Il6r  (interleukin 6 receptor)

Genes (Mus musculus)
Il6  (interleukin 6)
Il6ra  (interleukin 6 receptor, alpha)

Genes (Homo sapiens)
IL6  (interleukin 6)
IL6R  (interleukin 6 receptor)


Additional Information