RGD Reference Report - Synaptotagmin-12, a synaptic vesicle phosphoprotein that modulates spontaneous neurotransmitter release. - Rat Genome Database

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Synaptotagmin-12, a synaptic vesicle phosphoprotein that modulates spontaneous neurotransmitter release.

Authors: Maximov, A  Shin, OH  Liu, X  Sudhof, TC 
Citation: Maximov A, etal., J Cell Biol. 2007 Jan 1;176(1):113-24. Epub 2006 Dec 26.
RGD ID: 10047304
Pubmed: PMID:17190793   (View Abstract at PubMed)
PMCID: PMC2063632   (View Article at PubMed Central)
DOI: DOI:10.1083/jcb.200607021   (Journal Full-text)

Central synapses exhibit spontaneous neurotransmitter release that is selectively regulated by cAMP-dependent protein kinase A (PKA). We now show that synaptic vesicles contain synaptotagmin-12, a synaptotagmin isoform that differs from classical synaptotagmins in that it does not bind Ca(2+). In synaptic vesicles, synaptotagmin-12 forms a complex with synaptotagmin-1 that prevents synaptotagmin-1 from interacting with SNARE complexes. We demonstrate that synaptotagmin-12 is phosphorylated by cAMP-dependent PKA on serine(97), and show that expression of synaptotagmin-12 in neurons increases spontaneous neurotransmitter release by approximately threefold, but has no effect on evoked release. Replacing serine(97) by alanine abolishes synaptotagmin-12 phosphorylation and blocks its effect on spontaneous release. Our data suggest that spontaneous synaptic-vesicle exocytosis is selectively modulated by a Ca(2+)-independent synaptotagmin isoform, synaptotagmin-12, which is controlled by cAMP-dependent phosphorylation.




Biological Process

  
Object Symbol
Species
Term
Qualifier
Evidence
With
Notes
Source
Original Reference(s)
PrkacaRatpeptidyl-serine phosphorylation involved_inIMP PMID:17190793UniProt 
PrkacaRatspontaneous exocytosis of neurotransmitter involved_inIMP PMID:17190793UniProt 
Syt12Ratspontaneous exocytosis of neurotransmitter involved_inIDA PMID:17190793GO_Central 

Cellular Component

  
Object Symbol
Species
Term
Qualifier
Evidence
With
Notes
Source
Original Reference(s)
PrkacaRatpresynapse located_inIMP PMID:17190793UniProt 
Syt12Ratpresynapse located_inIDA PMID:17190793UniProt 
Syt12Ratsynaptic vesicle located_inIDA PMID:17190793UniProt 

Molecular Function

  
Object Symbol
Species
Term
Qualifier
Evidence
With
Notes
Source
Original Reference(s)
PrkacaRatcAMP-dependent protein kinase activity enablesIMP PMID:17190793UniProt 
Syt1Ratprotein binding enablesIPIUniProtKB:P97610PMID:17190793UniProt 
Syt12Ratprotein binding enablesIPIUniProtKB:P21707PMID:17190793UniProt 


Genes (Rattus norvegicus)
Prkaca  (protein kinase cAMP-activated catalytic subunit alpha) Syt1  (synaptotagmin 1) Syt12  (synaptotagmin 12)