RGD Reference Report - [A study of pulmonary inflammatory reaction induced by N-protein of SARS-CoV in rat models and effects of glucocorticoids on it]. - Rat Genome Database

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[A study of pulmonary inflammatory reaction induced by N-protein of SARS-CoV in rat models and effects of glucocorticoids on it].

Authors: Hao, Dong  He, Li-xian  Qu, Jie-ming  Pan, Jue  Hu, Bi-jie  Zhang, Jing  Li, Zhuo-zhe 
Citation: Hao D, etal., Zhonghua Nei Ke Za Zhi. 2005 Dec;44(12):890-3.
RGD ID: 32726073
Pubmed: PMID:16409721   (View Abstract at PubMed)


OBJECTIVE: To study the pulmonary inflammatory reaction induced by N-protein of SARS-CoV in rat models and the effects of glucocorticoids on the inflammatory reaction.
METHODS: The pulmonary inflammatory reaction in rat models were induced by intratracheal instillation of N-protein of SARS-CoV with a dose of 0.2 mg/kg. The rats were randomly divided into four groups: normal saline control group (Nc group), N-protein group 1 (P1 group, 6 h), N-protein group 2 (P2 group, 24 h), and N-protein + dexamethasone group (P + D group, dexamethasone 10 mg/kg intraperitoneally). The blood samples, bronchial alveolar lavage fluid (BALF) and lung tissue were collected after challenge. The cytological and histopathologic changes of lung tissues were observed and the wet/dry ratios (W/D) of lung tissue were determined. The interleukin (IL)-6, IL-10 and transforming growth factor-beta1 (TGF-beta1) of serum and BALF were measured by ELISA.
RESULTS: (1) Compared with the percentage of peripheral blood lymphocytes in Nc group [(68.42 +/- 13.07)%], that in P2 group [(50.50 +/- 14.36)%] was significantly decreased (P < 0.05); compared with Nc group and P2 group, that in P + D group was furthermore significantly decreased (P < 0.01). Compared with the total WBC of peripheral blood in Nc group [(5.86 +/- 2.25) x 10(9)] and P2 group [(4.83 +/- 1.49) x 10(9)], that in P + D group [(1.96 +/- 1.30) x 10(9)] was significantly decreased (P < 0.01). (2) Compared with the total WBC of BALF in Nc group [(95 +/- 29) x 10(7)], that in P2 group [(160 +/- 60) x 10(7)] was significantly increased (P < 0.05); but compared with P2 group, that in P + D group [(62 +/- 23) x 10(7)] was significantly decreased (P < 0.05). Analysis of BALF differential cell counts showed that the majority of cells were alveolar macrophages in all groups. (3) The W/D ratios of lung tissue in both P1 and P2 group [(5.18 +/- 0.29) and (5.19 +/- 0.34), respectively] after N-protein challenge were significantly increased than that in Nc groups [(4.77 +/- 0.27), P < 0.05]; the W/D ratio in P + D group (4.70 +/- 0.18) was significantly decreased than that in P2 group (P < 0.01). (4) Compared with Nc group, the levels of IL-6, IL-10, TGF-beta1 in both serum and BALF of P1 group were significantly increased (P < 0.01), and the levels of these cytokines in P2 group were significantly higher than those in P1 group (P < 0.01), but significantly lower in P + D group compared with P2 group (P < 0.01).
CONCLUSIONS: The N-protein of SARS-CoV had pathogenicity and could induce obvious pulmonary inflammatory reaction and acute lung injury, which were related to the increase and imbalance of pro-inflammatory and anti-inflammatory cytokines. Glucocorticoids could effectively alleviate the pulmonary inflammatory reaction induced by N-protein of SARS-CoV.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
severe acute respiratory syndrome  ISOIl10 (Rattus norvegicus)32726073; 32726073protein:increased expression:serum and bronchoalveolar Lavage fluid (rat)RGD 
severe acute respiratory syndrome  ISOIl6 (Rattus norvegicus)32726073; 32726073protein:increased expression:serum and Bronchoalveolar Lavage fluid (rat)RGD 
severe acute respiratory syndrome  IEP 32726073; 32726073protein:increased expression:serum and bronchoalveolar Lavage fluid (rat)RGD 
severe acute respiratory syndrome  IEP 32726073protein:increased expression:serum and Bronchoalveolar Lavage fluid (rat)RGD 
severe acute respiratory syndrome  ISOTgfb1 (Rattus norvegicus)32726073; 32726073protein:increased expression:serum and bronchoalveolar Lavage fluid (rat)RGD 

Objects Annotated

Genes (Rattus norvegicus)
Il10  (interleukin 10)
Il6  (interleukin 6)
Tgfb1  (transforming growth factor, beta 1)

Genes (Mus musculus)
Il10  (interleukin 10)
Il6  (interleukin 6)
Tgfb1  (transforming growth factor, beta 1)

Genes (Homo sapiens)
IL10  (interleukin 10)
IL6  (interleukin 6)
TGFB1  (transforming growth factor beta 1)


Additional Information