RGD Reference Report - Poly(ADP-ribose) polymerase-1 (PARP-1) gene deficiency alleviates diabetic kidney disease. - Rat Genome Database

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Poly(ADP-ribose) polymerase-1 (PARP-1) gene deficiency alleviates diabetic kidney disease.

Authors: Shevalye, H  Maksimchyk, Y  Watcho, P  Obrosova, IG 
Citation: Shevalye H, etal., Biochim Biophys Acta. 2010 Nov;1802(11):1020-7. doi: 10.1016/j.bbadis.2010.07.004. Epub 2010 Jul 16.
RGD ID: 11073737
Pubmed: PMID:20621183   (View Abstract at PubMed)
PMCID: PMC4217200   (View Article at PubMed Central)
DOI: DOI:10.1016/j.bbadis.2010.07.004   (Journal Full-text)

Poly(ADP-ribose)polymerase (PARP) inhibitors prevent or alleviate diabetic nephropathy. This study evaluated the role for PARP-1 in diabetic kidney disease using the PARP-1-deficient mouse. PARP-1-/- and the wild-type (129S1/SvImJ) mice were made diabetic with streptozotocin, and were maintained for 12 weeks. Final blood glucose concentrations were increased approximately 3.7-fold in both diabetic groups. PARP-1 protein expression (Western blot analysis) in the renal cortex was similar in non-diabetic and diabetic wild-type mice (100% and 107%) whereas all knockouts were PARP-1-negative. PARP-1 gene deficiency reduced urinary albumin (ELISA) and protein excretion prevented diabetes-induced kidney hypertrophy, and decreased mesangial expansion and collagen deposition (both assessed by histochemistry) as well as fibronectin expression. Renal podocyte loss (immunohistochemistry) and nitrotyrosine and transforming growth factor-beta(1) accumulations (both by ELISA) were slightly lower in diabetic PARP-1-/- mice, but the differences with diabetic wild-type group did not achieve statistical significance. In conclusion, PARP-1-/- gene deficiency alleviates although does not completely prevent diabetic kidney disease.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Diabetic Nephropathies  ISOParp1 (Mus musculus)11073737; 11073737associated with Diabetes Mellitus and ExperimentalRGD 
Diabetic Nephropathies  IMP 11073737associated with Diabetes Mellitus and ExperimentalRGD 

Objects Annotated

Genes (Rattus norvegicus)
Parp1  (poly (ADP-ribose) polymerase 1)

Genes (Mus musculus)
Parp1  (poly (ADP-ribose) polymerase family, member 1)

Genes (Homo sapiens)
PARP1  (poly(ADP-ribose) polymerase 1)


Additional Information