The homozygous Aire mutant rats are littermates of cross of heterozygotes Aire mutant rats . The mutations created by ZFN reagents targeting to a DNA sequence 59-TGCCACCCAGACCCCCCACAAAGAGAAGAGCCCTGGAAGAG-
39 in exon 3 of the
The BN homozygous Aire mutant rats were derived from founder rats that were backcrossed in a Sprague Dawley (SD; outbred strain) background for six generations to obtain AIRE-deficient SD rats. The original founder were from
BN mutants carrying the mutations created by ZFN reagents targeting to a DNA sequence 59-TGCCACCCAGACCCCCCACAAAGAGAAGAGCCCTGGAAGAG-
39 in exon 3 of the Aire gene. This mutant carriesa 17-bp deletion in the nuclear
localization signal sequence, causing a premature stop codon.
Descendants of rats from the Charles River Laboratories (USA),then to Fvet at Buenos Aires University and since 2014 maintained at Bioterio Central of Facultad de Farmacia y Bioquimica de la Universidad de Buenos Aires. Juni
Descendants of rats from the Wistar Institute, Philadelphia, Pennsylvania then to Harlan and now maintained at School of Science, Buenos Aires University, Ciudad Universitaria, Buenos Aires, Capital Federal, Argentina
Descendants of rats from the Wistar Institute, Philadelphia, Pennsylvania then to Harlan (Indianapolis).Since 1995 maintained at Bioterio Central- Facultad de Farmacia y Bioquÿ¿mica de la Universidad de Buenos Aires. Contact: Junin 956 8 piso Ciudad Autÿ¿nom