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Strain Registration

Strain: F344-Rag2em1Iexas

Symbol: F344-Rag2em1Iexas
Strain: F344-Rag2em1
Substrain: Iexas
RGD ID: 38599189
Citation ID: RRID:RGD_38599189
Ontology ID: RS:0004824
Alleles: Rag2em1Iexas
Also Known As: Rag2 KO; NBRP Rat No: 0894; F344-Rag2^[em1Iexas]
Type: mutant
Available Source: National BioResource Project for the Rat in Japan
Origination: National BioResource Project for the Rat in Japan
Description: This strain was established by targeting Rag2 gene in F344/Jcl using CRISPR/Cas9 system. gRNA to Rag2: AACATAGCCTTAATTCAACCAGG (PAM: last AGG); Cas 9 mRNA transcribed from T7-NLS hCas9-pA (RDB13130) was used for the system. Gene transfer was performed by electroporation. This strain shows severe combined immunodeficiency (SCID) caused by 1-bp insertion in Rag2 gene on chromosome 3. This strain grows normally under SPF condition.
Coat Color: albio
Last Known Status: Cryopreserved Embryo (as of 2020-09-14)
Research Usage immunology/Inflamation and Immunodeficient
Position
Rat AssemblyChrPosition (strand)SourceJBrowse
mRatBN7.2387,902,373 - 87,910,227RGD_MAPPER_PIPELINEmRatBN7.2
Rnor_6.0391,191,837 - 91,200,134RGD_MAPPER_PIPELINERnor6.0
Rnor_5.0397,851,318 - 97,859,615RGD_MAPPER_PIPELINERnor5.0
RGSC_v3.4386,764,810 - 86,773,438RGD_MAPPER_PIPELINERGSC3.4






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