RGD Reference Report - Regulation of kappa-opioid receptor mRNA level by cyclic AMP and growth factors in cultured rat glial cells. - Rat Genome Database

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Regulation of kappa-opioid receptor mRNA level by cyclic AMP and growth factors in cultured rat glial cells.

Authors: Tryoen-Toth, P  Gaveriaux-Ruff, C  Maderspach, K  Labourdette, G 
Citation: Tryoen-Toth P, etal., Brain Res Mol Brain Res. 1998 Mar 30;55(1):141-50.
RGD ID: 9831421
Pubmed: PMID:9645969   (View Abstract at PubMed)

The mRNA of the kappa-opioid receptor (KOR) has been found recently in cultured astrocytes and in microglia. By using RT-PCR and Southern hybridization, we confirmed these observations and, in addition, we observed that KOR mRNA was expressed in oligodendrocytes and in the precursors of astrocytes and oligodendrocytes. KOR mRNA level was the highest in the immature astrocytes and decreased with their maturation. Very few data are available on the regulation of KOR level by extracellular signals. Therefore, we examined the effect of three growth factors known to be present in the adult brain, basic fibroblast growth factor (bFGF), platelet-derived growth factor (PDGF-BB) and leukemia inhibitory factor (LIF) and of two cyclic AMP (cAMP) generating systems, the cAMP analog, 8-(4-chlorophenylthio)-cAMP and forskolin, on this level. It was found that in astrocytes, KOR mRNA level decreased dramatically under the effect of cAMP and less under the effect of bFGF while it did not change significantly after LIF treatment. In oligodendrocytes, it also decreased with cAMP, but increased under the effect of bFGF and PDGF-BB. In microglia, a decrease was observed with cAMP and lipopolysaccharides (LPS), the most used activators of macrophages. These results shed new evidence on the expression of opioid receptor mRNA in the glial cells of the rat CNS. The regulation of KOR mRNA level under the effect of extracellular signals suggests that opioids take part in dynamic processes in glial cells, possibly related to glial-neuron communication.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Oprk1Ratcellular response to lipopolysaccharide  IEP  RGD 
Oprk1Ratresponse to cocaine  IEP  RGD 
Oprk1Ratresponse to ethanol  IEP  RGD 
Oprk1Ratresponse to insulin  IEP  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Oprk1  (opioid receptor, kappa 1)


Additional Information