RGD Reference Report - Nitric oxide and carbon monoxide antagonize TGF-beta through ligand-independent internalization of TbetaR1/ALK5. - Rat Genome Database

Send us a Message
Submit Data |  Help |  Video Tutorials |  News |  Publications |  Download |  REST API |  Citing RGD |  Contact   
Search RGD Grant Resources Citing RGD About Us Contact Us
Genes Variants Community Projects QTLs Strains Markers Genome Information Ontologies Cell Lines References Download Submit Data
OntoMate (Literature Search) JBrowse (Genome Browser) Synteny Browser (VCMap) Variant Visualizer Multi-Ontology Enrichment (MOET) Gene-Ortholog Location Finder (GOLF) InterViewer (Protein-Protein Interactions) PhenoMiner (Quatitative Phenotypes) Gene Annotator OLGA (Gene List Generator) AllianceMine GViewer (Genome Viewer)
Aging & Age-Related Disease Cancer & Neoplastic Disease Cardiovascular Disease Coronavirus Disease Developmental Disease Diabetes Hematologic Disease Immune & Inflammatory Disease Infectious Disease Liver Disease Neurological Disease Obesity & Metabolic Syndrome Renal Disease Respiratory Disease Sensory Organ Disease
Find Models Genetic Models Autism Models Rat PhenoMiner (Quantitative Phenotypes) Chinchilla PhenoMiner Expected Ranges (Quantitative Phenotype) PhenoMiner Term Comparison Hybrid Rat Diversity Panel Phenotypes Phenotypes in Other Animal Models Animal Husbandry Strain Medical Records Phylogenetics Strain Availability Calendar Rats 101 Submissions Photo Archive
Pathways
Rat Community Forum Directory of Rat Laboratories Video Tutorials News RGD Publications RGD Presentations Archive Nomenclature Guidelines Resource Links Laboratory Resources Employment Resources
Advanced Search (OLGA)

Nitric oxide and carbon monoxide antagonize TGF-beta through ligand-independent internalization of TbetaR1/ALK5.

Authors: Hovater, MB  Ying, WZ  Agarwal, A  Sanders, PW 
Citation: Hovater MB, etal., Am J Physiol Renal Physiol. 2014 Sep 15;307(6):F727-35. doi: 10.1152/ajprenal.00353.2014. Epub 2014 Aug 6.
RGD ID: 9685289
Pubmed: PMID:25100282   (View Abstract at PubMed)
PMCID: PMC4166728   (View Article at PubMed Central)
DOI: DOI:10.1152/ajprenal.00353.2014   (Journal Full-text)

Transforming growth factor (TGF)-beta plays a central role in vascular homeostasis and in the pathology of vascular disease. There is a growing appreciation for the role of nitric oxide (NO) and carbon monoxide (CO) as highly diffusible, bioactive signaling molecules in the vasculature. We hypothesized that both NO and CO increase endocytosis of TGF-beta receptor type 1 (TbetaR1) in vascular smooth muscle cells (VSMCs) through activation of dynamin-2, shielding cells from the effects of circulating TGF-beta. In this study, primary cultures of VSMCs from Sprague-Dawley rats were treated with NO-releasing molecule 3 (a NO chemical donor), CO-releasing molecule 2 (a CO chemical donor), or control. NO and CO stimulated dynamin-2 activation in VSMCs. NO and CO promoted time- and dose-dependent endocytosis of TbetaR1. By decreasing TbetaR1 surface expression through this dynamin-2-dependent process, NO and CO diminished the effects of TGF-beta on VSMCs. These findings help explain an important mechanism by which NO and CO signal in the vasculature by decreasing surface expression of TbetaR1 and the cellular response to TGF-beta.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Dnm2Ratcellular response to carbon monoxide  IEP  RGD 
Dnm2Ratcellular response to nitric oxide  IEP  RGD 
Dnm2Ratnegative regulation of transforming growth factor beta receptor signaling pathway  IMP  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Dnm2  (dynamin 2)


Additional Information