RGD Reference Report - Abnormal DNA methylation in CD4+ T cells from people with latent autoimmune diabetes in adults. - Rat Genome Database

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Abnormal DNA methylation in CD4+ T cells from people with latent autoimmune diabetes in adults.

Authors: Li, Y  Zhao, M  Hou, C  Liang, G  Yang, L  Tan, Y  Wang, Z  Yin, H  Zhou, Z  Lu, Q 
Citation: Li Y, etal., Diabetes Res Clin Pract. 2011 Nov;94(2):242-8. doi: 10.1016/j.diabres.2011.07.027. Epub 2011 Aug 23.
RGD ID: 9589109
Pubmed: PMID:21864931   (View Abstract at PubMed)
DOI: DOI:10.1016/j.diabres.2011.07.027   (Journal Full-text)

Aberrant DNA methylation in T cells has been linked to pathogenesis of autoimmune diseases. To investigate genomic and gene-specific DNA methylation levels in CD4(+) T cells from patients with latent autoimmune diabetes in adults (LADA), and to investigate changes in the expression of genes that regulate methylation as well as the autoimmune-related gene FOXP3 in these patients. Global CD4(+) T cell DNA methylation was measured in 15 LADA patients and 11 healthy controls using a methylation quantification kit. mRNA levels of DNA methytransferases (DNMTs), methyl-DNA binding domain proteins (MBDs) and FOXP3 were measured by real time PCR. Methylation of a FOXP3 regulatory element region was determined by bisulphite genomic sequencing. Genomic DNA methylation in CD4(+) T cells from LADA patients was significantly increased compared to controls. DNMT3b mRNA levels were higher in CD4(+) T cells from LADA patients than in controls. DNMT3b expression positively correlated with global DNA methylation in LADA CD4(+) T cells. FOXP3 expression was decreased, and the FOXP3 promoter region was hypermethylated in CD4(+) T cells from LADA patients compared with controls. DNA methylation levels are altered in CD4(+) T cells from LADA patients, which may contribute to disease onset and progression by affecting the expression of autoimmune-related genes.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
DNMT3BHumantype 1 diabetes mellitus  IEP mRNA:increased expression:CD4+ T cell:RGD 
Dnmt3bRattype 1 diabetes mellitus  ISODNMT3B (Homo sapiens)mRNA:increased expression:CD4+ T cell:RGD 
Dnmt3bMousetype 1 diabetes mellitus  ISODNMT3B (Homo sapiens)mRNA:increased expression:CD4+ T cell:RGD 

Objects Annotated

Genes (Rattus norvegicus)
Dnmt3b  (DNA methyltransferase 3 beta)

Genes (Mus musculus)
Dnmt3b  (DNA methyltransferase 3B)

Genes (Homo sapiens)
DNMT3B  (DNA methyltransferase 3 beta)


Additional Information