RGD Reference Report - Effect of somatostatin on repair of ionizing radiation-induced DNA damage in pituitary adenoma cells GH3. - Rat Genome Database

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Effect of somatostatin on repair of ionizing radiation-induced DNA damage in pituitary adenoma cells GH3.

Authors: Rezacova, M  Cap, J  Vokurkova, D  Lukasova, E  Vavrova, J  Cerman, J  Masin, V  Mazankova, N 
Citation: Rezacova M, etal., Physiol Res. 2008;57(2):225-35. Epub 2007 May 30.
RGD ID: 8693710
Pubmed: PMID:17552875   (View Abstract at PubMed)

Ionizing radiation and somatostatin analogues are used for acromegaly treatment to achieve normalization or reduction of growth hormone hypersecretion and tumor shrinkage. In this study, we investigated a combination of somatostatin (SS14) with ionizing radiation of (60)Co and its effect on reparation of radiation-induced damage and cell death of somatomammotroph pituitary cells GH3. Doses of gamma-radiation 20-50 Gy were shown to inhibit proliferation and induce apoptosis in GH3 cells regardless of somatostatin presence. It has been found that the D(0) value for GH3 cells was 2.5 Gy. Somatostatin treatment increased radiosensitivity of GH3 cells, so that D(0) value decreased to 2.2 Gy. We detected quick phosphorylation of histone H2A.X upon irradiation by the dose 20 Gy and its colocalization with phosphorylated protein Nbs-1 in the site of double strand break of DNA (DSB). Number of DSB decreased significantly 24 h after irradiation, however, clearly distinguished foci persisted, indicating non repaired DSB, after irradiation alone or after combined treatment by irradiation and SS14. We found that SS14 alone triggers phosphorylation of Nbs1 (p-Nbs1), which correlates with antiproliferative effect of SS14. Irradiation also increased the presence of p-Nbs1. Most intensive phosphorylation of Nbs1 was detected after combined treatment of irradiation and SS14. The decrease of the number of the DSB foci 24 h after treatment shows a significant capacity of repair systems of GH3 cells. In spite of this, large number of unrepaired DSB persists for 24 h after the treatment. We conclude that SS14 does not have a radioprotective effect on somatomammotroph GH3 cells.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
H2axRatcellular response to gamma radiation  IDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
H2ax  (H2A.X variant histone)


Additional Information