RGD Reference Report - Involvement of RelA-associated inhibitor in regulation of trophoblast differentiation via interaction with transcriptional factor specificity protein-1. - Rat Genome Database

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Involvement of RelA-associated inhibitor in regulation of trophoblast differentiation via interaction with transcriptional factor specificity protein-1.

Authors: Minekawa, R  Sakata, M  Okamoto, Y  Hayashi, M  Isobe, A  Takeda, T  Yamamoto, T  Koyama, M  Ohmichi, M  Tasaka, K  Imai, K  Okamoto, T  Murata, Y 
Citation: Minekawa R, etal., Endocrinology. 2007 Dec;148(12):5803-10. Epub 2007 Sep 13.
RGD ID: 8693594
Pubmed: PMID:17872376   (View Abstract at PubMed)
DOI: DOI:10.1210/en.2007-0142   (Journal Full-text)

Glucose transporter-1 (GLUT1), one of the key functional indicators of placental differentiation, has an important role in placental glucose transport. We previously showed that the protein levels of GLUT1 and nuclear transcription factor specificity protein-1 (Sp1) in rat choriocarcinoma cells (Rcho-1 cells) decreased during the differentiation of these cells to giant cells. We also showed that Sp1 was involved in the regulation of GLUT1 gene expression during this process. RelA-associated inhibitor (RAI) is an inhibitor of nuclear factor-kappaB that was identified by a yeast two-hybrid screen and is preferably expressed in human placenta and heart. RAI was also found to interact with Sp1 and exert an inhibitory effect against the DNA-binding activity of Sp1. We first show here that RAI mRNA expression increased as gestation proceeded and that RAI was localized mainly in the syncytiotrophoblast throughout pregnancy. The chloramphenicol acetyltransferase activity assay in Rcho-1 cells revealed that cotransfection of RAI expression vector resulted in decreased activity of the rat GLUT1 promoter but not in that of a mutated rat GLUT1 promoter lacking the Sp1 binding site. Furthermore, the protein level of RAI increased during differentiation. In addition, transfection of RAI expression vector promoted the morphological differentiation of Rcho-1 cells, and RAI knockdown using RAI-specific small interfering RNA reveals inhibitory effects on the morphological differentiation, as assessed by photomicroscopy. Taken together, these findings suggest that RAI may be involved in the regulation of trophoblast differentiation via interaction with Sp1.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Ppp1r13lRatpositive regulation of cell differentiation  IMP  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Ppp1r13l  (protein phosphatase 1, regulatory subunit 13 like)


Additional Information