RGD Reference Report - Spexin expression in normal rat tissues. - Rat Genome Database

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Spexin expression in normal rat tissues.

Authors: Porzionato, A  Rucinski, M  Macchi, V  Stecco, C  Malendowicz, LK  De Caro, R 
Citation: Porzionato A, etal., J Histochem Cytochem. 2010 Sep;58(9):825-37. doi: 10.1369/jhc.2010.956300. Epub 2010 Jun 7.
RGD ID: 8693371
Pubmed: PMID:20530460   (View Abstract at PubMed)
PMCID: PMC2924798   (View Article at PubMed Central)
DOI: DOI:10.1369/jhc.2010.956300   (Journal Full-text)

Spexin is a highly conserved peptide which was recently identified through the bioinformatics approach. Immunohistochemical analysis of its expression has not yet been performed. Thus, in this study, we examined spexin location in a wide range of rat organs by both RT-PCR and IHC. RT-PCR identified spexin mRNA in all tissues examined. Spexin immunoreaction was mainly cytoplasmic. Spexin was immunohistochemically detected, although with different staining intensities, in epithelia and glands of skin and respiratory, digestive, urinary, and reproductive systems. Smooth muscle cells showed weak immunostaining, and connective tissue was negative. In the central nervous system, neuronal groups showed different intensities for reaction product. Immunoreaction was also found in ganglionic cells of both trigeminal and superior cervical ganglia and in photoreceptor, inner nuclear, and ganglionic layers of the retina. In the endocrine system, spexin immunoreaction was detected in the hypothalamic paraventricular and supraoptic nuclei; adenohypophysis, thyroid, and parathyroid glands; adrenal cortex and medulla (mainly ganglionic cells); Leydig cells; and thecal, luteal, and interstitial cells of the ovary. Because of its widespread expression, spexin is probably involved in many different physiological functions; in particular, location of spexin in neurons and endocrine cells suggests its roles as neurotransmitter/neuromodulator and endocrine factor.



Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
SpxRatcytoplasm located_inIDA PMID:20530460UniProt 

Objects Annotated

Genes (Rattus norvegicus)
Spx  (spexin hormone)


Additional Information