RGD Reference Report - [Inhibition effect of osthole on proliferation of rat chondrocytes]. - Rat Genome Database

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[Inhibition effect of osthole on proliferation of rat chondrocytes].

Authors: Ding, Dao-fang  Wei, Song-pu  Li, Xiao-feng  Zhang, Xiao-gang  Zhan, Hong-sheng  Duan, Tie-li  Cao, Yue-long 
Citation: Ding DF, etal., Zhong Xi Yi Jie He Xue Bao. 2012 Dec;10(12):1413-8. doi: 10.3736/jcim20121213.
RGD ID: 8657411
Pubmed: PMID:23257135   (View Abstract at PubMed)
DOI: DOI:10.3736/jcim20121213   (Journal Full-text)


OBJECTIVE: To investigate the effects of osthole on chondrocyte proliferation in vitro.
METHODS: Primary rat chondrocytes were isolated from the femoral head of newborn rats using collagenase digestion and cultured in Dulbecco's modified Eagle's medium. The proliferation of primary chondrocytes was assessed in second-passage cultures using cell counting kit-8 and the growth curve was drawn. Type II procollagen gene (Col2a1) expression in chondrocytes was also identified using cell immunofluorescence assay. The second-passage chondrocytes were divided into five groups, including control group and osthole groups at 6.25, 12.5, 25 and 50 µmol/L. The growth property of rat chondrocytes was observed after 24, 48 and 72 h of culture with osthole at corresponding dose. Both protein and mRNA expression of proliferating cell nuclear antigen (PCNA) and cyclin D1 was measured by Western blot and polymerase chain reaction methods.
RESULTS: The second-passage chondrocytes were viable and showed Col2a1 expression in the cytoplasm. The proliferation of rat chondrocytes was inhibited by osthole in a dose-dependent manner. Meanwhile, there were significant decreases in both protein and mRNA expression of PCNA and cyclin D1 in the osthole groups compared with the control group.
CONCLUSION: Osthole exhibits inhibitory effect on proliferation of rat chondrocytes by down-regulating PCNA and cyclin D1 expression.



Objects referenced in this article
Gene Col2a1 collagen type II alpha 1 chain Rattus norvegicus

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