RGD Reference Report - Survival response-linked Pyk2 activation during potassium depletion-induced apoptosis of cerebellar granule neurons. - Rat Genome Database

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Survival response-linked Pyk2 activation during potassium depletion-induced apoptosis of cerebellar granule neurons.

Authors: Strappazzon, F  Torch, S  Trioulier, Y  Blot, B  Sadoul, R  Verna, JM 
Citation: Strappazzon F, etal., Mol Cell Neurosci. 2007 Mar;34(3):355-65. Epub 2006 Dec 22.
RGD ID: 8554817
Pubmed: PMID:17188509   (View Abstract at PubMed)
DOI: DOI:10.1016/j.mcn.2006.11.012   (Journal Full-text)

Numerous extracellular stimuli trigger trans-autophosphorylation at Tyr402 of Pyk2, inducing its activation. Pyk2 is a key mediator of several signaling pathways and has been implicated in apoptosis induced by specific stress signals. We investigated whether Pyk2 participates in cerebellar granule neuron (CGN) apoptosis induced by the suppression of membrane depolarization. We demonstrate that shifting CGN cultures from 25 mM to 5 mM KCl-containing medium induces an early, transient 70% increase in phosphorylated Tyr402 and Tyr580 Pyk2 levels that is triggered by Ca(2+) released from intracellular stores and mediated by calmodulin (CaM). Overexpression of Pyk2 increases CGN survival after 24 h by 70% compared to the control, thus suggesting that Pyk2 is involved in an anti-apoptotic response to K+ lowering. Furthermore, we show that CGN grown in K25 medium exhibit detectable CaM-dependent Pyk2 activity. When silencing Pyk2 activity by expressing a dominant-negative form, only 40% of the transfected neurons were alive 24 h after transfection when compared to the control. Overall, the present findings demonstrate for the first time that Pyk2 is a critical mediator of CGN survival.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Ptk2bRatcellular defense response involved_inIMP PMID:17188509Alzheimers_University_of_Toronto 
Ptk2bRatnegative regulation of neuron apoptotic process involved_inIMP PMID:17188509Alzheimers_University_of_Toronto 
Ptk2bRatresponse to cation stress involved_inIDA PMID:17188509Alzheimers_University_of_Toronto 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Ptk2bRatcalcium/calmodulin-dependent protein kinase activity enablesIDA PMID:17188509Alzheimers_University_of_Toronto 
Ptk2bRatnon-membrane spanning protein tyrosine kinase activity enablesIDA PMID:17188509Alzheimers_University_of_Toronto 

Objects Annotated

Genes (Rattus norvegicus)
Ptk2b  (protein tyrosine kinase 2 beta)


Additional Information