RGD Reference Report - Cdk5 is involved in neuregulin-induced AChR expression at the neuromuscular junction. - Rat Genome Database

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Cdk5 is involved in neuregulin-induced AChR expression at the neuromuscular junction.

Authors: Fu, AK  Fu, WY  Cheung, J  Tsim, KW  Ip, FC  Wang, JH  Ip, NY 
Citation: Fu AK, etal., Nat Neurosci. 2001 Apr;4(4):374-81.
RGD ID: 8554712
Pubmed: PMID:11276227   (View Abstract at PubMed)
DOI: DOI:10.1038/86019   (Journal Full-text)

Here we describe an important involvement of Cdk5/p35 in regulating the gene expression of acetylcholine receptor (AChR) at the neuromuscular synapse. Cdk5 and p35 were prominently expressed in embryonic muscle, and concentrated at the neuromuscular junction in adulthood. Neuregulin increased the p35-associated Cdk5 kinase activity in the membrane fraction of cultured C2C12 myotubes. Co-immunoprecipitation studies revealed the association between Cdk5, p35 and ErbB receptors in muscle and cultured myotubes. Inhibition of Cdk5 activity not only blocked the NRG-induced AChR transcription, but also attenuated ErbB activation in cultured myotubes. In light of our finding that overexpression of p35 alone led to an increase in AChR promoter activity in muscle, Cdk5 activation is sufficient to mediate the up-regulation of AChR gene expression. Taken together, these results reveal the unexpected involvement of Cdk5/p35 in neuregulin signaling at the neuromuscular synapse.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Cdk5Rataxon extension involved_inTAS PMID:11276227UniProt 
Cdk5r1Rataxon extension involved_inTAS PMID:11276227UniProt 
Cdk5r1Ratembryo development ending in birth or egg hatching involved_inIDA PMID:11276227UniProt 
Cdk5r1RatG protein-coupled acetylcholine receptor signaling pathway involved_inIDA PMID:11276227UniProt 
Cdk5Ratskeletal muscle tissue development involved_inTAS PMID:11276227UniProt 

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Cdk5r1Ratcontractile muscle fiber located_inIDA PMID:11276227UniProt 
Cdk5Ratcytoplasm located_inIDA PMID:11276227UniProt 
Cdk5r1Ratcytoplasm located_inIDA PMID:11276227UniProt 
Cdk5Ratmembrane located_inIDA PMID:11276227UniProt 
Cdk5r1Ratmembrane located_inIDA PMID:11276227UniProt 
Cdk5Ratneuromuscular junction is_active_inIDA PMID:11276227SynGO 
Cdk5Ratneuromuscular junction located_inIDA PMID:11276227UniProt 
Cdk5r1Ratneuromuscular junction located_inIDA PMID:11276227UniProt 
Cdk5Ratpostsynapse is_active_inIDA PMID:11276227SynGO 

Objects Annotated

Genes (Rattus norvegicus)
Cdk5  (cyclin-dependent kinase 5)
Cdk5r1  (cyclin-dependent kinase 5 regulatory subunit 1)


Additional Information